ArticleThe Journal of cell biology2009
Quantitative analysis of chromatin compaction in living cells using FLIM-FRET.
Article in The Journal of cell biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 96 papers.
What it found
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Who cites it
96 citing papers in PubMed, 186 citations in OpenAlex.
- Benchmarking three simple DNA staining-based image metrics for live-cell tracking of chromatin organization.Nucleus (Austin, Tex.) · 2026Article
- Multiscale modeling primer: Focus on chromatin and epigenetics.The Journal of biological chemistry · 2026Review
- HIRA-SETDB1-H3K9me3 axis regulates chromatin architecture in leukemia cells.The Journal of biological chemistry · 2026Article
- The Extremely Low Mechanical Force Generated by Nano-Pulling Induces Global Changes in the Microtubule Network, Nuclear Morphology, and Chromatin Transcription in Neurons.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Calyculin A Induces Premature Chromosome Condensation and Chromatin Compaction in GbioRxiv : the preprint server for biology · 2025Article
- High-speed fluorescence lifetime imaging microscopy: techniques, applications, and prospects.Biophotonics discovery · 2025Review
- The DEAD-box helicase eIF4A1/2 acts as RNA chaperone during mitotic exit enabling chromatin decondensation.Nature communications · 2025Article
- Decreased DNA density is a better indicator of a nuclear bleb than lamin B loss.Journal of cell science · 2025Article
- Near millimolar concentration of nucleosomes in mitotic chromosomes from late prometaphase into anaphase.The Journal of cell biology · 2024Article
- Ion-mediated condensation controls the mechanics of mitotic chromosomes.Nature materials · 2024Article
- Heterochromatin protein 1 alpha (HP1α) undergoes a monomer to dimer transition that opens and compacts live cell genome architecture.Nucleic acids research · 2024Article
- Probing chromatin condensation dynamics in live cells using interferometric scattering correlation spectroscopy.Communications biology · 2024Article
- Opticool: Cutting-edge transgenic optical tools.PLoS genetics · 2024Review
- Article
- Super-resolution microscopy reveals the nanoscale cluster architecture of the DEK protein cancer biomarker.iScience · 2023Article
- Multi-Scale Imaging of the Dynamic Organization of Chromatin.International journal of molecular sciences · 2023Review
- Nuclear lamina strain states revealed by intermolecular force biosensor.Nature communications · 2023Article
- Evidence for low nanocompaction of heterochromatin in living embryonic stem cells.The EMBO journal · 2023Article
- Evaluation by Experimentation and Simulation of a FRET Pair Comprising Fluorescent Nucleobase Analogs in Nucleosomes.Chemistry (Weinheim an der Bergstrasse, Germany) · 2023Article
- Extending fluorescence anisotropy to large complexes using reversibly switchable proteins.Nature biotechnology · 2023Article
36 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
We present a quantitative Förster resonance energy transfer (FRET)-based assay using multiphoton fluorescence lifetime imaging microscopy (FLIM) to measure chromatin compaction at the scale of nucleosomal arrays in live cells. The assay uses a human cell line coexpressing histone H2B tagged to either enhanced green fluorescent protein (FP) or mCherry FPs (HeLa(H2B-2FP)). FRET occurs between FP-tagged histones on separate nucleosomes and is increased when chromatin compacts. Interphase cells consistently show three populations of chromatin with low, medium, or high FRET efficiency, reflecting spatially distinct regions with different levels of chromatin compaction. Treatment with inhibitors that either increase chromatin compaction (i.e., depletion of adenosine triphosphate) or decrease chromosome compaction (trichostatin A) results in a parallel increase or decrease in the FLIM-FRET signal. In mitosis, the assay showed variation in compaction level, as reflected by different FRET efficiency populations, throughout the length of all chromosomes, increasing to a maximum in late anaphase. These data are consistent with extensive higher order folding of chromatin fibers taking place during anaphase.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.