ArticleBMC neuroscience2009
Dynamic visualization of membrane-inserted fraction of pHluorin-tagged channels using repetitive acidification technique.
Article in BMC neuroscience, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Neuroactive steroids activate membrane progesterone receptors to induce sex specific effects on protein kinase activity.iScience · 2025Article
- Progress in pH-Sensitive sensors: essential tools for organelle pH detection, spotlighting mitochondrion and diverse applications.Frontiers in pharmacology · 2023Review
- Organelle-specific single-molecule imaging of α4β2 nicotinic receptors reveals the effect of nicotine on receptor assembly and cell-surface trafficking.The Journal of biological chemistry · 2017Article
- Ammonium chloride alters neuronal excitability and synaptic vesicle release.Scientific reports · 2017Article
- Utilizing pHluorin-tagged Receptors to Monitor Subcellular Localization and Trafficking.Journal of visualized experiments : JoVE · 2017Article
- Article
- Identification of the SNARE complex mediating the exocytosis of NMDA receptors.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Nicotinic Acetylcholine Receptors as Targets for Tobacco Cessation Therapeutics: Cutting-Edge Methodologies to Understand Receptor Assembly and Trafficking.Neuromethods · 2016Article
- The nicotine metabolite, cotinine, alters the assembly and trafficking of a subset of nicotinic acetylcholine receptors.The Journal of biological chemistry · 2015Article
- Lynx1 shifts α4β2 nicotinic receptor subunit stoichiometry by affecting assembly in the endoplasmic reticulum.The Journal of biological chemistry · 2014Article
- Illumination of the spatial order of intracellular pH by genetically encoded pH-sensitive sensors.Sensors (Basel, Switzerland) · 2013Review
- Defining the structural relationship between kainate-receptor deactivation and desensitization.Nature structural & molecular biology · 2013Article
- investigating acid production by Streptococcus mutans with a surface-displayed pH-sensitive green fluorescent protein.PloS one · 2013Article
- Splice isoform estrogen receptors as integral transmembrane proteins.Molecular biology of the cell · 2011Article
- Characterizing functional α6β2 nicotinic acetylcholine receptors in vitro: mutant β2 subunits improve membrane expression, and fluorescent proteins reveal responsive cells.Biochemical pharmacology · 2011Article
- Unusually Strong Temperature Dependence of P2X3 Receptor Traffic to the Plasma Membrane.Frontiers in cellular neuroscience · 2011Article
- Nicotine up-regulates alpha4beta2 nicotinic receptors and ER exit sites via stoichiometry-dependent chaperoning.The Journal of general physiology · 2011Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundChanges in neuronal excitability, synaptic efficacy and generally in cell signaling often result from insertion of key molecules into plasma membrane (PM). Many of the techniques used for monitoring PM insertion lack either spatial or temporal resolution.
resultsWe improved the imaging method based on time-lapse total internal reflection fluorescence (TIRF) microscopy and pHluorin tagging by supplementing it with a repetitive extracellular acidification protocol. We illustrate the applicability of this method by showing that brief activation of NMDA receptors ("chemical LTP") in cultured hippocampal neurons induced a persistent PM insertion of glutamate receptors containing the pHluorin-tagged GluR-A(flip) subunits.
conclusionThe repetitive acidification technique provides a more accurate way of monitoring the PM-inserted fraction of fluorescently tagged molecules and offers a good temporal and spatial resolution.
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