ArticleJournal of Zhejiang University. Science. B2009
Optimization of soybean (Glycine max (L.) Merrill) in planta ovary transformation using a linear minimal gus gene cassette.
Article in Journal of Zhejiang University. Science. B, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed.
- A fast and genotype-independent in planta Agrobacterium-mediated transformation method for soybean.Plant communications · 2024Article
- Advances in Soybean Genetic Improvement.Plants (Basel, Switzerland) · 2024Review
- A comprehensive review of in planta stable transformation strategies.Plant methods · 2024Review
- Crop genome editing through tissue-culture-independent transformation methods.Frontiers in genome editing · 2024Review
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4 authors.
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Abstract
Soybean transformation by ovary-drip was improved by optimizing the length of the transformation pathway by cutting the styles. These modifications facilitated soybean transformation manipulation and improved transformation reproducibility and efficiency. Using a linear minimal gus gene cassette as the foreign DNA, a maximum transformation frequency of 11% was obtained in flowers of the soybean cultivar 'Liaodou 14' with their styles mostly removed, whereas removal of only the stigma, partial style cutting and partial ovary cutting gave transformation frequencies of 0%, 1%, and 2%, respectively. An average transformation frequency of 8.2% was obtained when 619 flowers from three soybean cultivars ('Liaodou 14', 'Liaodou 13', and 'Tiefeng 29') were transformed by this optimized method. Southern blotting analysis showed that the gus reporter gene (encoding beta-glucuronidase) was stably inherited with a simple pattern. Reverse transcription-polymerase chain reaction (RT-PCR) and GUS staining confirmed the expression of the gus gene in transgenic plants.
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