ArticleGlycobiology2010
A motif-based analysis of glycan array data to determine the specificities of glycan-binding proteins.
Article in Glycobiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed.
- GlycanGT: a pretrained graph transformer framework for glycan graph representation and generative learning.Bioinformatics (Oxford, England) · 2026Article
- The energetic landscape of CH-π interactions in protein-carbohydrate binding.Chemical science · 2025Article
- CarboGrove: a resource of glycan-binding specificities through analyzed glycan-array datasets from all platforms.Glycobiology · 2022Article
- Combined Analysis of Multiple Glycan-Array Datasets: New Explorations of Protein-Glycan Interactions.Analytical chemistry · 2021Article
- Microarray analyses of closely related glycoforms reveal different accessibilities of glycan determinants on N-glycan branches.Glycobiology · 2020Article
- Identifying glycan motifs using a novel subtree mining approach.BMC bioinformatics · 2020Article
- Advances in Tools to Determine the Glycan-Binding Specificities of Lectins and Antibodies.Molecular & cellular proteomics : MCP · 2020Review
- GLAD: GLycan Array Dashboard, a visual analytics tool for glycan microarrays.Bioinformatics (Oxford, England) · 2019Article
- Glycointeractions in bacterial pathogenesis.Nature reviews. Microbiology · 2018Review
- Applying Pose Clustering and MD Simulations To Eliminate False Positives in Molecular Docking.Journal of chemical information and modeling · 2018Article
- Mining High-Complexity Motifs in Glycans: A New Language To Uncover the Fine Specificities of Lectins and Glycosidases.Analytical chemistry · 2017Article
- Characterizing Protein Glycosylation through On-Chip Glycan Modification and Probing.Analytical chemistry · 2016Article
- Article
- Combining 3D structure with glycan array data provides insight into the origin of glycan specificity.Glycobiology · 2016Article
- Lack of phosphomannomutase 2 affects Xenopus laevis morphogenesis and the non-canonical Wnt5a/Ror2 signalling.Journal of inherited metabolic disease · 2015Article
- Association analyses of large-scale glycan microarray data reveal novel host-specific substructures in influenza A virus binding glycans.Scientific reports · 2015Article
- Upregulation of glycans containing 3' fucose in a subset of pancreatic cancers uncovered using fusion-tagged lectins.Journal of proteome research · 2015Article
- Glycan motif profiling reveals plasma sialyl-lewis x elevations in pancreatic cancers that are negative for sialyl-lewis A.Molecular & cellular proteomics : MCP · 2015Article
- The detection and discovery of glycan motifs in biological samples using lectins and antibodies: new methods and opportunities.Advances in cancer research · 2015Review
- Exploring the specificities of glycan-binding proteins using glycan array data and the GlycoSearch software.Methods in molecular biology (Clifton, N.J.) · 2015Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Glycan arrays have enabled detailed studies of the specificities of glycan-binding proteins. A challenge in the interpretation of glycan array data is to determine the specific features of glycan structures that are critical for binding. To address this challenge, we have developed a systematic method to interpret glycan array data using a motif-based analysis. Each glycan on a glycan array is classified according to its component sub-structures, or motifs. We analyze the binding of a given lectin to each glycan in terms of the motifs in order to identify the motifs that are selectively present in the glycans that are bound by the lectin. We compared two different methods to calculate the identification, termed intensity segregation and motif segregation, for the analysis of three well-characterized lectins with highly divergent behaviors. Both methods accurately identified the primary specificities as well as the weaker, secondary specificities of all three lectins. The complex binding behavior of wheat germ agglutinin was reduced to its simplified, independent specificities. We compiled the motif specificities of a wide variety of plant lectins, human lectins, and glycan-binding antibodies to uncover the relationships among the glycan-binding proteins and to provide a means to search for lectins with particular binding specificities. This approach should be valuable for rapidly analyzing and using glycan array data, for better describing and understanding glycan-binding specificities, and as a means to systematize and compare data from glycan arrays.
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Registered trials
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