ArticleJournal of reproductive immunology2010
Identification of human sperm proteins that interact with human zona pellucida3 (ZP3) using yeast two-hybrid system.
Article in Journal of reproductive immunology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 33 citations in OpenAlex.
- The Use of Yeast Two-Hybrid Analysis to Study Protein-Protein Interactions for Ustilago maydis.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Functional Methods for Studying Sperm-Zona Pellucida Interaction in Mammals.Methods and protocols · 2025Review
- Whole-exome sequencing identified a novel heterozygous variant inFrontiers in genetics · 2024Article
- UBAP2L promotes gastric cancer metastasis by activating NF-κB through PI3K/AKT pathway.Cell death discovery · 2022Article
- Ubiquitin Binding Protein 2-Like (UBAP2L): is it so NICE After All?Frontiers in cell and developmental biology · 2022Review
- UBAP2L arginine methylation by PRMT1 modulates stress granule assembly.Cell death and differentiation · 2020Article
- Upregulation of UBAP2L in Bone Marrow Mesenchymal Stem Cells Promotes Functional Recovery in Rats with Spinal Cord Injury.Current medical science · 2018Article
- UBA2 promotes proliferation of colorectal cancer.Molecular medicine reports · 2018Article
- Knockdown of Ubiquitin Associated Protein 2-Like (UBAP2L) Inhibits Growth and Metastasis of Hepatocellular Carcinoma.Medical science monitor : international medical journal of experimental and clinical research · 2018Article
- Screening and structural and functional investigation of a novel ferritin from Phascolosoma esculenta.Protein science : a publication of the Protein Society · 2017Article
- Depletion of UBA protein 2-like protein inhibits growth and induces apoptosis of human colorectal carcinoma cells.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Article
- Article
- Downregulation of ubiquitin-associated protein 2-like with a short hairpin RNA inhibits human glioma cell growth in vitro.International journal of molecular medicine · 2015Article
- The role of SPRASA in female fertility.Reproductive sciences (Thousand Oaks, Calif.) · 2015Article
- The RNA-binding proteins FMR1, rasputin and caprin act together with the UBA protein lingerer to restrict tissue growth in Drosophila melanogaster.PLoS genetics · 2013Article
- The Aryl-Hydrocarbon Receptor Protein Interaction Network (AHR-PIN) as Identified by Tandem Affinity Purification (TAP) and Mass Spectrometry.Journal of toxicology · 2013Article
- Antisperm contraceptive vaccines: where we are and where we are going?American journal of reproductive immunology (New York, N.Y. : 1989) · 2011Review
- Sperm proteasomes degrade sperm receptor on the egg zona pellucida during mammalian fertilization.PloS one · 2011Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Sperm proteins that interact with zona pellucida 3 (ZP3) have not been clearly identified in humans. In the present study, the yeast two-hybrid (Y2H) system was used to identify human sperm proteins that interact with human ZP3. Human ZP3 cDNA was cloned into pAS2-1 yeast vector and used as bait to find reactive proteins in the human testis cDNA library. Six specific clones were obtained that were further confirmed for interaction using the mammalian two-hybrid system. These six clones showed homologies with several proteins in the GenBank database. Of these, the strongest ZP3-interacting protein, that shows 97% homology with ubiquitin associated protein-2 like (UBAP2L), was tested in the hemizona assay. UBAP2L antibodies significantly (p<0.001) inhibited human sperm-zona binding in this assay. We conclude that the Y2H system is a useful strategy for identifying novel genes encoding proteins that interact with ZP proteins. To our knowledge, this is the first study using the Y2H system to identify sperm proteins that interact with human oocyte ZP3. Novel proteins identified using this system may find applications in elucidating the fertilization cascade, development of a new generation of non-steroidal contraceptives, and specific diagnosis and treatment of human infertility.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.