ArticleBMC genomics2009
Alu-directed transcriptional regulation of some novel miRNAs.
Article in BMC genomics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 55 citations in OpenAlex.
- Perspective for Studying the Relationship of miRNAs with Transposable Elements.Current issues in molecular biology · 2023Article
- Alu RNA and their roles in human disease states.RNA biology · 2021Article
- Article
- Genome-wide analysis of polymerase III-transcribedGenome research · 2019Article
- Human Survival Motor Neuron genes generate a vast repertoire of circular RNAs.Nucleic acids research · 2019Article
- Review
- Decoding the Emerging Patterns Exhibited in Non-coding RNAs Characteristic of Lung Cancer with Regard to their Clinical Significance.Current genomics · 2018Article
- Exploratory bioinformatics investigation reveals importance of "junk" DNA in early embryo development.BMC genomics · 2017Article
- A Multilayered Control of the HumanFrontiers in microbiology · 2017Review
- Alu repeats as transcriptional regulatory platforms in macrophage responses to M. tuberculosis infection.Nucleic acids research · 2016Article
- Alu-miRNA interactions modulate transcript isoform diversity in stress response and reveal signatures of positive selection.Scientific reports · 2016Article
- Alu retrotransposons promote differentiation of human carcinoma cells through the aryl hydrocarbon receptor.Nucleic acids research · 2016Article
- Human Ribosomal RNA-Derived Resident MicroRNAs as the Transmitter of Information upon the Cytoplasmic Cancer Stress.BioMed research international · 2016Article
- Short interspersed DNA elements and miRNAs: a novel hidden gene regulation layer in zebrafish?Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2015Article
- Analysis of 13 cell types reveals evidence for the expression of numerous novel primate- and tissue-specific microRNAs.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Article
- Genome-Wide Identification and Classification of MicroRNAs Derived from Repetitive Elements.Genomics & informatics · 2014Article
- The long non-coding RNA PCAT-1 promotes prostate cancer cell proliferation through cMyc.Neoplasia (New York, N.Y.) · 2014Article
- Dynamic Alu methylation during normal development, aging, and tumorigenesis.BioMed research international · 2014Review
- Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDespite many studies on the biogenesis, molecular structure and biological functions of microRNAs, little is known about the transcriptional regulatory mechanisms controlling the spatiotemporal expression pattern of human miRNA gene loci. Several lines of experimental results have indicated that both polymerase II (Pol-II) and polymerase III (Pol-III) may be involved in transcribing miRNAs. Here, we assessed the genomic evidence for Alu-directed transcriptional regulation of some novel miRNA genes in humans. Our data demonstrate that the expression of these Alu-related miRNAs may be modulated by Pol-III.
resultsWe present a comprehensive exploration of the Alu-directed transcriptional regulation of some new miRNAs. Using a new computational approach, a variety of Alu-related sequences from multiple sources were pooled and filtered to obtain a subset containing Alu elements and characterized miRNA genes for which there is clear evidence of full-length transcription (embedded in EST). We systematically demonstrated that 73 miRNAs including five known ones may be transcribed by Pol-III through Alu or MIR. Among the new miRNAs, 33 were determined by high-throughput Solexa sequencing. Real-time TaqMan PCR and Northern blotting verified that three newly identified miRNAs could be induced to co-express with their upstream Alu transcripts by heat shock or cycloheximide.
conclusionThrough genomic analysis, Solexa sequencing and experimental validation, we have identified candidate sequences for Alu-related miRNAs, and have found that the transcription of these miRNAs could be governed by Pol-III. Thus, this study may elucidate the mechanisms by which the expression of a class of small RNAs may be regulated by their upstream repeat elements.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.