ArticleBMC microbiology2009
Comparison of the sensitivity of culture, PCR and quantitative real-time PCR for the detection of Pseudomonas aeruginosa in sputum of cystic fibrosis patients.
Article in BMC microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.
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Who cites it
30 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Prevalence of vancomycin-resistant enterococcus in Africa in one health approach: a systematic review and meta-analysis.Scientific reports · 2020Pooled it
- Antibacterial and antibiofilm properties of 1,3,4-oxadiazoles against multidrug-resistant Pseudomonas aeruginosa isolated from burn infections - an in vitro study.International microbiology : the official journal of the Spanish Society for Microbiology · 2026Article
- Molecular endotyping in people with bronchiectasis based on response to antibiotic treatment: iBEST study.ERJ open research · 2025Article
- Comparative evaluation of proteinase K and dithiothreitol as pretreatments for extracting nucleic acids from respiratory samples for multiplex PCR.BMC microbiology · 2025Article
- Rapid and sensitive isolation ofMicrobiology spectrum · 2025Article
- The airway microbiome of persons with cystic fibrosis correlates with acquisition and microbiological outcomes of incidentFrontiers in microbiology · 2024Article
- Antibacterial and Anti-Efflux Activities of Cinnamon Essential Oil against Pan and Extensive Drug-ResistantAntibiotics (Basel, Switzerland) · 2023Article
- PreventingACS applied bio materials · 2021Article
- A Loop-mediated Isothermal Amplification With a Nanoparticle-Based Lateral Flow Biosensor Assay to Detect Pseudomonas aeruginosa in Endophthalmitis.Translational vision science & technology · 2021Article
- Pneumocystis jirovecii among patients with cystic fibrosis and their household members.Medical mycology · 2021Observational
- Contamination of Hospital Surfaces with Bacterial Pathogens under the Current COVID-19 Outbreak.International journal of environmental research and public health · 2021Article
- Application of next generation sequencing-based rapid detection platform for microbiological diagnosis and drug resistance prediction in acute lower respiratory infection.Annals of translational medicine · 2020Article
- Fast and Accurate Pneumocystis Pneumonia Diagnosis in Human Samples Using a Label-Free Plasmonic Biosensor.Nanomaterials (Basel, Switzerland) · 2020Article
- Digital PCR as an Emerging Tool for Monitoring of Microbial Biodegradation.Molecules (Basel, Switzerland) · 2020Review
- Comparison of OmpA Gene-Targeted Real-Time PCR with the Conventional Culture Method for Detection of Acinetobacter baumanii in Pneumonic BALB/c MiceIranian biomedical journal · 2019Article
- Detection of viable but non-culturable Pseudomonas aeruginosa in cystic fibrosis by qPCR: a validation study.BMC infectious diseases · 2018Article
- A bimodal (SERS and colorimetric) aptasensor for the detection of Pseudomonas aeruginosa.Mikrochimica acta · 2018Article
- Recent Advances in Molecular Diagnosis ofFrontiers in microbiology · 2018Article
- Macrophage migration inhibitory factor enhancesFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2017Article
- Molecular Identification of Staphylococcus aureus in Airway Samples from Children with Cystic Fibrosis.PloS one · 2016Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPseudomonas aeruginosa is the major pathogen involved in the decline of lung function in cystic fibrosis (CF) patients. Early aggressive antibiotic therapy has been shown to be effective in preventing chronic colonization. Therefore, early detection is important and sensitive detection methods are warranted. In this study, we used a dilution series of P. aeruginosa positive sputa, diluted in a pool of P. aeruginosa negative sputa, all from CF patients--to mimick as closely as possible the sputa sent to routine laboratories--to compare the sensitivity of three culture techniques versus that of two conventional PCR formats and four real-time PCR formats, each targeting the P. aeruginosa oprL gene. In addition, we compared five DNA-extraction protocols.
resultsIn our hands, all three culture methods and the bioMérieux easyMAG Nuclisens protocol Generic 2.0.1, preceded by proteinase K pretreatment and followed by any of the 3 real-time PCR formats with probes were most sensitive and able to detect P. aeruginosa up to 50 cfu/ml, i.e. the theoretical minimum of one cell per PCR mixture, when taking into account the volumes used in this study of sample for DNA-extraction, of DNA-elution and of DNA-extract in the PCR mixture.
conclusionIn this study, no difference in sensitivity could be found for the detection of P. aeruginosa from sputum between microbiological culture and optimized DNA-extraction and real-time PCR. The results also indicate the importance of the optimization of the DNA-extraction protocol and the PCR format.
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