ArticleMolecular microbiology2010
Autonomous plasmid-like replication of a conjugative transposon.
Article in Molecular microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 78 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
78 citing papers in PubMed, 120 citations in OpenAlex.
- Two OB-fold proteins from a Gram-positive conjugative element engage in relaxosome assembly and DNA processing.Nucleic acids research · 2025Article
- Bacterial evolution in the oral microbiome: the role of conjugative elements and horizontal gene transfer.Journal of bacteriology · 2025Review
- The extended mobility of plasmids.Nucleic acids research · 2025Review
- Interaction with eukaryotic cells enhances the circularization and transcription of integrative and conjugative elements in Mycoplasma hominis.Mobile DNA · 2025Article
- Activation and modulation of the host response to DNA damage by an integrative and conjugative element.Journal of bacteriology · 2025Article
- Mobile genetic elements in shapingFrontiers in microbiology · 2025Review
- Activation and modulation of the host response to DNA damage by an integrative and conjugative element.bioRxiv : the preprint server for biology · 2024Article
- Article
- Recipient UvrD helicase is involved in single- to double-stranded DNA conversion during conjugative plasmid transfer.Nucleic acids research · 2023Article
- Timing of integration into the chromosome is critical for the fitness of an integrative and conjugative element and its bacterial host.PLoS genetics · 2023Article
- Establishing genetic manipulation for novel strains of human gut bacteria.Microbiome research reports · 2023Review
- SEN1990 is a predicted winged helix-turn-helix protein involved in the pathogenicity ofFrontiers in microbiology · 2023Article
- TnSmu1 is a functional integrative and conjugative element in Streptococcus mutans that when expressed causes growth arrest of host bacteria.Molecular microbiology · 2022Article
- Second-Generation Transfer Mediates Efficient Propagation of ICEJournal of bacteriology · 2022Article
- Selfish, promiscuous and sometimes useful: how mobile genetic elements drive horizontal gene transfer in microbial populations.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Review
- Exploration of DNA processing features unravels novel properties of ICE conjugation in Gram-positive bacteria.Nucleic acids research · 2022Article
- A CRISPR interference screen reveals a role for cell wall teichoic acids in conjugation in Bacillus subtilis.Molecular microbiology · 2022Article
- Biology and engineering of integrative and conjugative elements: Construction and analyses of hybrid ICEs reveal element functions that affect species-specific efficiencies.PLoS genetics · 2022Article
- Article
- IncC helper dependent plasmid-like replication of Salmonella Genomic Island 1.Nucleic acids research · 2021Article
18 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Integrative and conjugative elements (ICEs), a.k.a. conjugative transposons, are mobile genetic elements involved in many biological processes, including pathogenesis, symbiosis and the spread of antibiotic resistance. Unlike conjugative plasmids that are extra-chromosomal and replicate autonomously, ICEs are integrated in the chromosome and replicate passively during chromosomal replication. It is generally thought that ICEs do not replicate autonomously. We found that when induced, Bacillus subtilis ICEBs1 undergoes autonomous plasmid-like replication. Replication was unidirectional, initiated from the ICEBs1 origin of transfer, oriT, and required the ICEBs1-encoded relaxase NicK. Replication also required several host proteins needed for chromosomal replication, but did not require the replicative helicase DnaC or the helicase loader protein DnaB. Rather, replication of ICEBs1 required the helicase PcrA that is required for rolling circle replication of many plasmids. Transfer of ICEBs1 from the donor required PcrA, but did not require replication, indicating that PcrA, and not DNA replication, facilitates unwinding of ICEBs1 DNA for horizontal transfer. Although not needed for horizontal transfer, replication of ICEBs1 was needed for stability of the element. We propose that autonomous plasmid-like replication is a common property of ICEs and contributes to the stability and maintenance of these mobile genetic elements in bacterial populations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.