ArticleThe EMBO journal2009
Cullin neddylation and substrate-adaptors counteract SCF inhibition by the CAND1-like protein Lag2 in Saccharomyces cerevisiae.
Article in The EMBO journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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Who cites it
22 citing papers in PubMed, 35 citations in OpenAlex.
- CAND1 mediates CUL7-dependent HER2 protein stability to drive breast cancer progression.Breast cancer research : BCR · 2025Article
- A synthetic lethal screen identifies HDAC4 as a potential target in MELK overexpressing cancers.G3 (Bethesda, Md.) · 2021Article
- Neddylation inhibitor MLN4924 induces GOncology letters · 2018Article
- Cullin 3-Based Ubiquitin Ligases as Master Regulators of Mammalian Cell Differentiation.Trends in biochemical sciences · 2018Review
- Trade-off and flexibility in the dynamic regulation of the cullin-RING ubiquitin ligase repertoire.PLoS computational biology · 2017Article
- The devil is in the details: comparison between COP9 signalosome (CSN) and the LID of the 26S proteasome.Current genetics · 2016Review
- Skp1 Independent Function of Cdc53/Cul1 in F-box Protein Homeostasis.PLoS genetics · 2015Article
- The NEDD8 modification pathway in plants.Frontiers in plant science · 2014Review
- Structural and functional insights to ubiquitin-like protein conjugation.Annual review of biophysics · 2014Review
- Article
- CSN- and CAND1-dependent remodelling of the budding yeast SCF complex.Nature communications · 2013Article
- CAND1 controls in vivo dynamics of the cullin 1-RING ubiquitin ligase repertoire.Nature communications · 2013Article
- Structural basis for a reciprocal regulation between SCF and CSN.Cell reports · 2012Article
- Neddylation and CAND1 independently stimulate SCF ubiquitin ligase activity in Candida albicans.Eukaryotic cell · 2012Article
- The TFIIH subunit Tfb3 regulates cullin neddylation.Molecular cell · 2011Article
- Notch-induced Asb2 expression promotes protein ubiquitination by forming non-canonical E3 ligase complexes.Cell research · 2011Article
- Non-SCF-type F-box protein Roy1/Ymr258c interacts with a Rab5-like GTPase Ypt52 and inhibits Ypt52 function.Molecular biology of the cell · 2011Article
- New insight into the role of the Cdc34 ubiquitin-conjugating enzyme in cell cycle regulation via Ace2 and Sic1.Genetics · 2011Article
- Recruitment of the inhibitor Cand1 to the cullin substrate adaptor site mediates interaction to the neddylation site.Molecular biology of the cell · 2011Article
- The essential functions of NEDD8 are mediated via distinct surface regions, and not by polyneddylation in Schizosaccharomyces pombe.PloS one · 2011Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 4 countries.
Funding
Abstract
Cullin-based E3 ubiquitin ligases are activated through covalent modification of the cullin subunit by the ubiquitin-like protein Nedd8. Cullin neddylation dissociates the ligase assembly inhibitor Cand1, and promotes E2 recruitment and ubiquitin transfer by inducing a conformational change. Here, we have identified and characterized Lag2 as a likely Saccharomyces cerevisiae orthologue of mammalian Cand1. Similar to Cand1, Lag2 directly interacts with non-neddylated yeast cullin Cdc53 and prevents its neddylation in vivo and in vitro. Binding occurs through a conserved C-terminal beta-hairpin structure that inserts into the Skp1-binding pocket on the cullin, and an N-terminal motif that covers the neddylation lysine. Interestingly, Lag2 is itself neddylated in vivo on a lysine adjacent to this N-terminal-binding site. Overexpression of Lag2 inhibits Cdc53 activity in strains defective for Skp1 or neddylation functions, implying that these activities are important to counteract Lag2 in vivo. Our results favour a model in which binding of substrate-specific adaptors triggers release of Cand1/Lag2, whereas subsequent neddylation of the cullin facilitates the removal and prevents re-association of Lag2/Cand1.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.