ArticleJournal of the American Society for Mass Spectrometry2010
What does the future hold for Top Down mass spectrometry?
Article in Journal of the American Society for Mass Spectrometry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed.
- Mass Spectrometry-Based Identification of Post-Translational Modifications (PTMs) of Proteins.Advances in experimental medicine and biology · 2026Article
- Post-translational modifications of vertebrate striated muscle myosin heavy chains.Cytoskeleton (Hoboken, N.J.) · 2024Review
- Expert Algorithm for Substance Identification Using Mass Spectrometry: Statistical Foundations in Unimolecular Reaction Rate Theory.Journal of the American Society for Mass Spectrometry · 2023Article
- Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing (Frog) Embryo.Journal of visualized experiments : JoVE · 2022Article
- Fourier-transform ion cyclotron resonance mass spectrometry for characterizing proteoforms.Mass spectrometry reviews · 2022Review
- Standard procedures for native CZE-MS of proteins and protein complexes up to 800 kDa.Electrophoresis · 2021Article
- TopPIC Gateway: A Web Gateway for Top-Down Mass Spectrometry Data Interpretation.PEARC20 : Practice and Experience in Advanced Research Computing 2020 : Catch the wave : July 27-31, 2020, Portland, Or Virtual Conference. Practice and Experience in Advanced Research Computing (Conference) (2020 : Online) · 2020Article
- Quantitation of Single and Combinatorial Histone Modifications by Integrated Chromatography of Bottom-up Peptides and Middle-down Polypeptide Tails.Journal of the American Society for Mass Spectrometry · 2019Article
- Unambiguous Sequence Characterization of a Monoclonal Antibody in a Single Analysis Using a Nonspecific Immobilized Enzyme Reactor.Analytical chemistry · 2019Article
- Middle-down approach: a choice to sequence and characterize proteins/proteomes by mass spectrometry.RSC advances · 2018Review
- Optimisation of Milk Protein Top-Down Sequencing Using In-Source Collision-Induced Dissociation in the Maxis Quadrupole Time-of-Flight Mass Spectrometer.Molecules (Basel, Switzerland) · 2018Article
- Maillard Proteomics: Opening New Pages.International journal of molecular sciences · 2017Review
- Analysis of Proteins, Protein Complexes, and Organellar Proteomes Using Sheathless Capillary Zone Electrophoresis - Native Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2017Article
- Front-End Electron Transfer Dissociation Coupled to a 21 Tesla FT-ICR Mass Spectrometer for Intact Protein Sequence Analysis.Journal of the American Society for Mass Spectrometry · 2017Article
- Informed-Proteomics: open-source software package for top-down proteomics.Nature methods · 2017Article
- Phosphoproteomics with Activated Ion Electron Transfer Dissociation.Analytical chemistry · 2017Article
- High Mass Ion Detection with Charge Detector Coupled to Rectilinear Ion Trap Mass Spectrometer.Journal of the American Society for Mass Spectrometry · 2017Article
- Complete Characterization of Cardiac Myosin Heavy Chain (223 kDa) Enabled by Size-Exclusion Chromatography and Middle-Down Mass Spectrometry.Analytical chemistry · 2017Article
- Monitoring proteolytic processing events by quantitative mass spectrometry.Expert review of proteomics · 2017Review
- Toward an Optimized Workflow for Middle-Down Proteomics.Analytical chemistry · 2017Article
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Abstract
Mass spectrometry (MS) research has revolutionized modern biological and biomedical fields. At the heart of the majority of mass spectrometry experiments is the use of Bottom Up mass spectrometry methods where proteins are first proteolyzed into smaller fragments before MS interrogation. The advent of electron capture dissociation and, more recently, electron-transfer dissociation, however, has allowed Top Down (analysis of intact proteins) or middle down (analysis of large polypeptides) mass spectrometry to both experience large increases in development, growth, and overall usage. Nevertheless, for high-throughput large-scale proteomic studies, Bottom Up mass spectrometry has easily dominated the field. As Top Down mass spectrometry methodology and technology continue to develop, will it genuinely be able to compete with Bottom Up mass spectrometry for whole proteome analysis? Discussed here are the current approaches, applications, issues, and future view of high-throughput Top Down mass spectrometry.
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Registered trials
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