ArticleVirology journal2009
High genotypic diversity and a novel variant of human cytomegalovirus revealed by combined UL33/UL55 genotyping with broad-range PCR.
Article in Virology journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 17 citations in OpenAlex.
- Feasibility of using saliva for Cytomegalovirus detection and genotyping in pediatric hematopoietic stem cell transplant recipients.Acta odontologica latinoamericana : AOL · 2025Article
- Why Are Cytomegalovirus-Encoded G-Protein-Coupled Receptors Essential for Infection but Only Variably Conserved?Pathogens (Basel, Switzerland) · 2025Review
- Molecular Detection and Glycoprotein B (UL55) Genotyping of Cytomegalovirus among Sudanese Renal Transplant Recipients.BioMed research international · 2022Article
- Pre-existing immunity to cytomegalovirus in macaques influences human CMV vaccine responses in preclinical models.Vaccine · 2021Article
- Human Cytomegalovirus Genomes Sequenced Directly From Clinical Material: Variation, Multiple-Strain Infection, Recombination, and Gene Loss.The Journal of infectious diseases · 2019Article
- Human cytomegalovirus genomics and transcriptomics through the lens of next-generation sequencing: revision and future challenges.Virus genes · 2019Review
- Positively Selected Sites at HCMV gB Furin Processing Region and Their Effects in Cleavage Efficiency.Frontiers in microbiology · 2017Article
- Strain Variation and Disease Severity in Congenital Cytomegalovirus Infection: In Search of a Viral Marker.Infectious disease clinics of North America · 2015Review
- High-throughput analysis of human cytomegalovirus genome diversity highlights the widespread occurrence of gene-disrupting mutations and pervasive recombination.Journal of virology · 2015Article
- Combination of immunoglobulins and natural killer cells in the context of CMV and EBV infection.Medical microbiology and immunology · 2014Article
- Antiviral function and efficacy of polyvalent immunoglobulin products against CMV isolates in different human cell lines.Medical microbiology and immunology · 2012Article
- A novel bat herpesvirus encodes homologues of major histocompatibility complex classes I and II, C-type lectin, and a unique family of immune-related genes.Journal of virology · 2012Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The known strains of human cytomegalovirus (HCMV) represent genotypic variants of a single species, and HCMV genotypic variability has been studied in order to reveal correlations between different disease patterns and the presence of certain HCMV genotypes, either as single or as multiple infections. The methods used for the detection of HCMV genotypes have not always been sophisticated enough to achieve complete comprehensiveness, mainly because only one genotype is usually detected in a certain specimen, due to primer specificity and genome copy number. To improve detection of variant HCMV genotypes in mixed infections, we developed PCR assays with degenerate primers targeting two variable HCMV genes, glycoprotein B (gB, UL55) and the G-protein-coupled receptor gene UL33. Primers were designed to bind conserved sites in the genomes of HCMV variants and great ape CMVs. To analyse if samples contained one or more HCMV genotypic variants, PCR assays were supplemented with oligonucleotides containing locked nucleic acids. This broad-range PCR methodology and subsequent sequence analysis detected all gB/UL55 and UL33 genotypic variants known to date in primary clinical specimens, but also revealed that many samples contained genotype mixtures. Importantly, a novel UL33 genotypic variant could be discovered in several specimens, and one HCMV isolate was plaque-purified containing the novel UL33 genotype and a so far undescribed variant of gB.
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