ArticleProceedings of the National Academy of Sciences of the United States of America2009
A two-subunit bacterial sigma-factor activates transcription in Bacillus subtilis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 15 citations in OpenAlex.
- Chimeric MerR-Family Regulators and Logic Elements for the Design of Metal Sensitive Genetic Circuits inACS synthetic biology · 2023Article
- Extracytoplasmic Function σ Factors as Tools for Coordinating Stress Responses.International journal of molecular sciences · 2021Review
- Expansion and re-classification of the extracytoplasmic function (ECF) σ factor family.Nucleic acids research · 2021Article
- ComWΔ6 Stimulates Transcription of Pneumococcal Competence GenesFrontiers in molecular biosciences · 2020Article
- Where to begin? Sigma factors and the selectivity of transcription initiation in bacteria.Molecular microbiology · 2019Review
- The pneumococcal σThe Journal of biological chemistry · 2019Article
- Characterization of a protein-protein interaction within the SigO-RsoA two-subunit σ factor: the σ70 region 2.3-like segment of RsoA mediates interaction with SigO.Microbiology (Reading, England) · 2016Article
- Promoter Escape with Bacterial Two-component σ Factor Suggests Retention of σ Region Two in the Elongation Complex.The Journal of biological chemistry · 2015Article
- Evolution of multisubunit RNA polymerases in the three domains of life.Nature reviews. Microbiology · 2011Review
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The sigma-like factor YvrI and coregulator YvrHa activate transcription from a small set of conserved promoters in Bacillus subtilis. We report here that these two proteins independently contribute sigma-region 2 and sigma-region 4 functions to a holoenzyme-promoter DNA complex. YvrI binds RNA polymerase (RNAP) through a region 4 interaction with the beta-subunit flap domain and mediates specific promoter recognition but cannot initiate DNA melting at the -10 promoter element. Conversely, YvrHa possesses sequence similarity to a conserved core-binding motif in sigma-region 2 and binds to the N-terminal coiled-coil element in the RNAP beta'-subunit previously implicated in interaction with region 2 of sigma-factors. YvrHa plays an essential role in stabilizing the open complex and interacts specifically with the N-terminus of YvrI. Based on these results, we propose that YvrHa is situated in the transcription complex proximal to the -10 element of the promoter, whereas YvrI is responsible for -35 region recognition. This system presents an unusual example of a two-subunit bacterial sigma-factor.
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Registered trials
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