ArticleThe Journal of biological chemistry2010
Myosin motors drive long range alignment of actin filaments.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 121 citations in OpenAlex.
- Elucidating chiral myosin-induced actin dynamics: From single-filament behavior to collective structures.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Emergence of diverse patterns driven by molecular motors in the motility assay.Cytoskeleton (Hoboken, N.J.) · 2024Article
- Improved longevity of actomyosin in vitro motility assays for sustainable lab-on-a-chip applications.Scientific reports · 2024Article
- Characterization of Mechanical and Cellular Effects of Rhythmic Vertical Vibrations on Adherent Cell Cultures.Bioengineering (Basel, Switzerland) · 2023Article
- Activity-induced polar patterns of filaments gliding on a sphere.Nature communications · 2022Article
- Noncentral forces mediated between two inclusions in a bath of active Brownian rods.Scientific reports · 2021Article
- Polar pattern formation induced by contact following locomotion in a multicellular system.eLife · 2020Article
- Synchronous operation of biomolecular engines.Biophysical reviews · 2020Review
- Article
- Effect of F-actin and Microtubules on Cellular Mechanical Behavior Studied Using Atomic Force Microscope and an Image Recognition-Based Cytoskeleton Quantification Approach.International journal of molecular sciences · 2020Article
- Mobility of Molecular Motors Regulates Contractile Behaviors of Actin Networks.Biophysical journal · 2019Article
- Myosin IIA Regulated Tight Junction in Oxygen Glucose-Deprived Brain Endothelial Cells Via Activation of TLR4/PI3K/Akt/JNK1/2/14-3-3ε/NF-κB/MMP9 Signal Transduction Pathway.Cellular and molecular neurobiology · 2019Article
- Architectural Dynamics of CaMKII-Actin Networks.Biophysical journal · 2019Article
- Millimeter-sized belt-like pattern formation of actin filaments in solution by interacting with surface myosinBiophysics and physicobiology · 2019Article
- Comparative analysis of widely used methods to remove nonfunctional myosin heads for the in vitro motility assay.Journal of muscle research and cell motility · 2018Article
- Rapid time-stamped analysis of filament motility.Journal of muscle research and cell motility · 2018Article
- Nanotopography-responsive myotube alignment and orientation as a sensitive phenotypic biomarker for Duchenne Muscular Dystrophy.Biomaterials · 2018Article
- Active nematics.Nature communications · 2018Review
- Self-organization across scales: from molecules to organisms.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Review
- DNA-assisted swarm control in a biomolecular motor system.Nature communications · 2018Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The bulk alignment of actin filament sliding movement, powered by randomly oriented myosin molecules, has been observed and studied using an in vitro motility assay. The well established, actin filament gliding assay is a minimal experimental system for studying actomyosin motility. Here, we show that when the assay is performed at densities of actin filaments approaching those found in living cells, filament gliding takes up a preferred orientation. The oriented patterns of movement that we have observed extend over a length scale of 10-100 microm, similar to the size of a mammalian cell. We studied the process of filament alignment and found that it depends critically upon filament length and density. We developed a simple quantitative measure of filament sliding orientation and this enabled us to follow the time course of alignment and the formation and disappearance of oriented domains. Domains of oriented filaments formed spontaneously and were separated by distinct boundaries. The pattern of the domain structures changed on the time scale of several seconds and the collision of neighboring domains led to emergence of new patterns. Our results indicate that actin filament crowding may play an important role in structuring the leading edge of migrating cells. Filament alignment due to near-neighbor mechanical interactions can propagate over a length scale of several microns; much greater than the size of individual filaments and analogous to a log drive. Self-alignment of actin filaments may make an important contribution to cell polarity and provide a mechanism by which cell migration direction responds to chemical cues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.