ArticleJournal of clinical microbiology2010
High-throughput pooling and real-time PCR-based strategy for malaria detection.
Article in Journal of clinical microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 100 papers, 4 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
100 citing papers in PubMed, 4 syntheses or guidelines pooled it.
- Overreliance on Plasmodium 18S rRNA gene for malaria molecular diagnosis-inferences from systematic review.Malaria journal · 2025Pooled it
- Molecular assays for determining sulphadoxine-pyrimethamine drug resistance in India: a systematic review.Parasitology research · 2022Pooled it
- A systematic review of sub-microscopic Plasmodium vivax infection.Malaria journal · 2015Pooled it
- Systematic review and meta-analysis: rapid diagnostic tests versus placental histology, microscopy and PCR for malaria in pregnant women.Malaria journal · 2011Pooled it
- Population pharmacokinetics of artesunate and dihydroartemisinin in pregnant and non-pregnant women with malaria.Malaria journal · 2011Trial
- Point of care performance of the improved BIOCREDIT Ag Pf/Pv (pLDH/pLDH) rapid diagnostic test: a cross-sectional study at a health center setting in Ethiopia.Malaria journal · 2026Article
- Comparative performance of microscopy, rapid diagnostic tests, and multiplex real-time PCR for detection of malaria parasites among pregnant women in northwest Ethiopia.Malaria journal · 2025Article
- Evaluation of Fecal Sample Pooling for Real-Time RT-PCR Testing SARS-CoV-2 in Animals.Viruses · 2024Article
- Diagnosis and management of malaria in the intensive care unit.Journal of intensive medicine · 2024Review
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- Article
- Use of compressed sensing to expedite high-throughput diagnostic testing for COVID-19 and beyond.PLoS computational biology · 2022Article
- Article
- Understanding the epidemiology, clinical characteristics, knowledge and barriers to treatment and prevention of malaria among returning international laborers in northern Vietnam: a mixed-methods study.BMC infectious diseases · 2022Article
- freqpcr: Estimation of population allele frequency using qPCR ΔΔCq measures from bulk samples.Molecular ecology resources · 2022Article
- Smart pooling: AI-powered COVID-19 informative group testing.Scientific reports · 2022Article
- Malaria diagnostic update: From conventional to advanced method.Journal of clinical laboratory analysis · 2022Review
- Rapid Visual Detection of Plasmodium Using Recombinase-Aided Amplification With Lateral Flow Dipstick Assay.Frontiers in cellular and infection microbiology · 2022Article
- Pooled Testing Strategies for SARS-CoV-2 diagnosis: A comprehensive review.Diagnostic microbiology and infectious disease · 2021Review
- A review of testing and assurance methods forFood and waterborne parasitology · 2021Article
40 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
Molecular assays can provide critical information for malaria diagnosis, speciation, and drug resistance, but their cost and resource requirements limit their application to clinical malaria studies. This study describes the application of a resource-conserving testing algorithm employing sample pooling for real-time PCR assays for malaria in a cohort of 182 pregnant women in Kinshasa. A total of 1,268 peripheral blood samples were collected during the study. Using a real-time PCR assay that detects all Plasmodium species, microscopy-positive samples were amplified individually; the microscopy-negative samples were amplified after pooling the genomic DNA (gDNA) of four samples prior to testing. Of 176 microscopy-positive samples, 74 were positive by the real-time PCR assay; the 1,092 microscopy-negative samples were initially amplified in 293 pools, and subsequently, 35 samples were real-time PCR positive (3%). With the real-time PCR result as the referent standard, microscopy was 67.9% sensitive (95% confidence interval [CI], 58.3% to 76.5%) and 91.2% specific (95% CI, 89.4% to 92.8%) for malaria. In total, we detected 109 parasitemias by real-time PCR and, by pooling samples, obviated over 50% of reactions and halved the cost of testing. Our study highlights both substantial discordance between malaria diagnostics and the utility and parsimony of employing a sample pooling strategy for molecular diagnostics in clinical and epidemiologic malaria studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.