ArticleClinical and vaccine immunology : CVI2010
Validation and clinical application of a molecular method for identification of Histoplasma capsulatum in human specimens in Colombia, South America.
Article in Clinical and vaccine immunology : CVI, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 55 citations in OpenAlex.
- Radiological features of chronic pulmonary histoplasmosis: Easily mistaken for tuberculosis.PLoS neglected tropical diseases · 2025Review
- Miliary Patterns on Computerized Tomography Imaging of Chest in Immunocompromised Patient: Unveiling Disseminated Histoplasmosis.The American journal of case reports · 2025Article
- Histoplasma capsulatum urinary antigen detection in a kidney transplant recipient with acute paracoccidioidomycosis: Case study and literature review.PLoS neglected tropical diseases · 2024Review
- Performance of rapid on-site evaluation of touch imprints of bronchoscopic biopsies or lung tissue biopsies for the diagnosis of invasive pulmonary filamentous fungi infections in non-neutropenic patients.Journal of clinical microbiology · 2024Article
- Insights on Covid-19 with superimposed pulmonary histoplasmosis: The possible nexus.Immunity, inflammation and disease · 2023Review
- Application of Real-Time PCR Assays for the Diagnosis of Histoplasmosis in Human FFPE Tissues Using Three Molecular Targets.Journal of fungi (Basel, Switzerland) · 2023Article
- Acute Pulmonary Histoplasmosis Following COVID-19: Novel Laboratorial Methods Aiding Diagnosis.Journal of fungi (Basel, Switzerland) · 2021Article
- Diagnostic accuracy of antigen detection in urine and molecular assays testing in different clinical samples for the diagnosis of progressive disseminated histoplasmosis in patients living with HIV/AIDS: A prospective multicenter study in Mexico.PLoS neglected tropical diseases · 2021Article
- Diagnosis of histoplasmosis: current status and perspectives.Applied microbiology and biotechnology · 2021Review
- Frequency of Invasive Fungal Disease in Adults: Experience of a Specialized Laboratory in Medellín, Colombia (2009-2015).Journal of fungi (Basel, Switzerland) · 2020Article
- The Diagnostic Laboratory Hub: A New Health Care System Reveals the Incidence and Mortality of Tuberculosis, Histoplasmosis, and Cryptococcosis of PWH in Guatemala.Open forum infectious diseases · 2020Article
- The Role of Molecular Tests in the Diagnosis of Disseminated Histoplasmosis.Journal of fungi (Basel, Switzerland) · 2019Review
- Histoplasmosis and Tuberculosis Co-Occurrence in People with Advanced HIV.Journal of fungi (Basel, Switzerland) · 2019Review
- Loop-mediated Isothermal Amplification and nested PCR of the Internal Transcribed Spacer (ITS) for Histoplasma capsulatum detection.PLoS neglected tropical diseases · 2019Article
- Usefulness of molecular markers in the diagnosis of occupational and recreational histoplasmosis outbreaks.Folia microbiologica · 2017Article
- Article
- Laryngeal histoplasmosis: report of the first case in Colombia.Colombia medica (Cali, Colombia) · 2015Article
- Implementation of a Training Course Increased the Diagnosis of Histoplasmosis in Colombia.The American journal of tropical medicine and hygiene · 2015Article
- Sarcoidosis and Histoplasmosis: Is One a Consequence of the Other? A Case Report and Review of the Literature.Case reports in rheumatology · 2015Article
- Development of a loop-mediated isothermal amplification method for detection of Histoplasma capsulatum DNA in clinical samples.Journal of clinical microbiology · 2014Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The conventional means of diagnosis of histoplasmosis presents difficulties because of the delay to the time that the diagnosis is made, indicating the need for the implementation of molecular assays. We evaluated 146 clinical samples from 135 patients suspected of having histoplasmosis using a previously reported nested PCR assay for the Histoplasma capsulatum-specific 100-kDa protein (the Hc100 PCR). In order to determine the specificity of this molecular test, we also used samples from healthy individuals (n = 20), patients suspected of having respiratory disease with negative fungal cultures (n = 29), and patients with other proven infections (n = 60). Additionally, a sizable collection of DNA from cultures of H. capsulatum and other medically relevant pathogens was studied. A panfungal PCR assay that amplified the internal transcribed spacer 2 region was also used to identify all fungal DNAs. All PCR-amplified products were sequenced. Of the 146 clinical samples, 67 (45.9%) were positive by culture and PCR, while 9 samples negative by culture were positive by PCR. All the sequences corresponding to the 76 amplified products presented > or =98% identity with H. capsulatum. The Hc100 PCR exhibited a sensitivity of 100% and specificities of 92.4% and 95.2% when the results were compared to those for the negative controls and samples from other proven clinical entities, respectively; the positive predictive value was 83% and the negative predictive value was 100%; the positive and negative likelihood rates were 25 and 0, respectively. These results suggest that the Hc100 nested PCR assay for the detection of H. capsulatum DNA is a useful test in areas where mycosis caused by this organism is endemic.
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