ArticleMolecular biology of the cell2010
Fast microtubule dynamics in meiotic spindles measured by single molecule imaging: evidence that the spindle environment does not stabilize microtubules.
Article in Molecular biology of the cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed.
- Article
- Microtubule choreography: spindle self-organization during cell division.Biophysical reviews · 2024Review
- A nucleotide binding-independent role for γ-tubulin in microtubule capping and cell division.The Journal of cell biology · 2023Article
- Morphological growth dynamics, mechanical stability, and active microtubule mechanics underlying spindle self-organization.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Article
- Review
- Gradual compaction of the central spindle decreases its dynamicity in PRC1 and EB1 gene-edited cells.Life science alliance · 2021Article
- Article
- Tension promotes kinetochore-microtubule release by Aurora B kinase.The Journal of cell biology · 2021Article
- Chemical tools for dissecting cell division.Nature chemical biology · 2021Review
- The coordination of spindle-positioning forces during the asymmetric division of the Caenorhabditis elegans zygote.EMBO reports · 2021Article
- Spatial variation of microtubule depolymerization in large asters.Molecular biology of the cell · 2021Article
- The tubulin code and its role in controlling microtubule properties and functions.Nature reviews. Molecular cell biology · 2020Review
- Mechanism of Long-Range Chromosome Motion Triggered by Gene Activation.Developmental cell · 2020Article
- Augmin accumulation on long-lived microtubules drives amplification and kinetochore-directed growth.The Journal of cell biology · 2019Article
- Article
- A computational model of the early stages of acentriolar meiotic spindle assembly.Molecular biology of the cell · 2019Article
- Measuring microtubule dynamics.Essays in biochemistry · 2018Review
- Article
- C. elegans chromosomes connect to centrosomes by anchoring into the spindle network.Nature communications · 2017Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Metaphase spindles are steady-state ensembles of microtubules that turn over rapidly and slide poleward in some systems. Since the discovery of dynamic instability in the mid-1980s, models for spindle morphogenesis have proposed that microtubules are stabilized by the spindle environment. We used single molecule imaging to measure tubulin turnover in spindles, and nonspindle assemblies, in Xenopus laevis egg extracts. We observed many events where tubulin molecules spend only a few seconds in polymer and thus are difficult to reconcile with standard models of polymerization dynamics. Our data can be quantitatively explained by a simple, phenomenological model-with only one adjustable parameter-in which the growing and shrinking of microtubule ends is approximated as a biased random walk. Microtubule turnover kinetics did not vary with position in the spindle and were the same in spindles and nonspindle ensembles nucleated by Tetrahymena pellicles. These results argue that the high density of microtubules in spindles compared with bulk cytoplasm is caused by local enhancement of nucleation and not by local stabilization. It follows that the key to understanding spindle morphogenesis will be to elucidate how nucleation is spatially controlled.
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Registered trials
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