ArticleRNA (New York, N.Y.)2010
Multiple determinants within iron-responsive elements dictate iron regulatory protein binding and regulatory hierarchy.
Article in RNA (New York, N.Y.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed, 71 citations in OpenAlex.
- Review
- SIREs 3.0, an improved RNA prediction tool for iron-responsive elements.Nucleic acids research · 2025Article
- Iron-Mediated Overexpression of Amyloid Precursor Protein via Iron Responsive mRNA in Alzheimer's Disease.International journal of molecular sciences · 2025Review
- Mathematical modeling reveals ferritin as the strongest cellular driver of dietary iron transfer block in enterocytes.PLoS computational biology · 2025Article
- α-Synuclein Iron-Responsive-Element RNA and Iron Regulatory Protein Affinity Is Specifically Reduced by Iron in Parkinson's Disease.Biomolecules · 2025Article
- Iron responsive elements mRNA regulate Alzheimer's amyloid precursor protein translation through iron sensing.Frontiers in aging neuroscience · 2025Review
- Targeting Iron Responsive Elements (IREs) of APP mRNA into Novel Therapeutics to Control the Translation of Amyloid-β Precursor Protein in Alzheimer's Disease.Pharmaceuticals (Basel, Switzerland) · 2024Review
- A temporal difference in the stabilization of two mRNAs with a 3' iron-responsive element during iron deficiency.RNA (New York, N.Y.) · 2023Article
- Iron-dependent post transcriptional control of mitochondrial aconitase expression.Metallomics : integrated biometal science · 2023Article
- Evaluation of candidate reference genes for quantitative real-time PCR analysis in a male rat model of dietary iron deficiency.Genes & nutrition · 2021Article
- Review
- Differential translational control of 5' IRE-containing mRNA in response to dietary iron deficiency and acute iron overload.Metallomics : integrated biometal science · 2020Article
- Iron-responsive-like elements and neurodegenerative ferroptosis.Learning & memory (Cold Spring Harbor, N.Y.) · 2020Review
- Article
- Targeting the Iron-Response Elements of the mRNAs for the Alzheimer's Amyloid Precursor Protein and Ferritin to Treat Acute Lead and Manganese Neurotoxicity.International journal of molecular sciences · 2019Review
- Manganese causes neurotoxic iron accumulation via translational repression of amyloid precursor protein and H-Ferritin.Journal of neurochemistry · 2018Article
- A synergistic role of IRP1 and FBXL5 proteins in coordinating iron metabolism during cell proliferation.The Journal of biological chemistry · 2017Article
- Mitochondrial iron chelation ameliorates cigarette smoke-induced bronchitis and emphysema in mice.Nature medicine · 2016Article
- Tethered Function Assays as Tools to Elucidate the Molecular Roles of RNA-Binding Proteins.Advances in experimental medicine and biology · 2016Article
- RNA-Binding Proteins in Trichomonas vaginalis: Atypical Multifunctional Proteins.Biomolecules · 2015Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Iron regulatory proteins (IRPs) are iron-regulated RNA binding proteins that, along with iron-responsive elements (IREs), control the translation of a diverse set of mRNA with 5' IRE. Dysregulation of IRP action causes disease with etiology that may reflect differential control of IRE-containing mRNA. IREs are defined by a conserved stem-loop structure including a midstem bulge at C8 and a terminal CAGUGH sequence that forms an AGU pseudo-triloop and N19 bulge. C8 and the pseudo-triloop nucleotides make the majority of the 22 identified bonds with IRP1. We show that IRP1 binds 5' IREs in a hierarchy extending over a ninefold range of affinities that encompasses changes in IRE binding affinity observed with human L-ferritin IRE mutants. The limits of this IRE binding hierarchy are predicted to arise due to small differences in binding energy (e.g., equivalent to one H-bond). We demonstrate that multiple regions of the IRE stem not predicted to contact IRP1 help establish the binding hierarchy with the sequence and structure of the C8 region displaying a major role. In contrast, base-pairing and stacking in the upper stem region proximal to the terminal loop had a minor role. Unexpectedly, an N20 bulge compensated for the lack of an N19 bulge, suggesting the existence of novel IREs. Taken together, we suggest that a regulatory binding hierarchy is established through the impact of the IRE stem on the strength, not the number, of bonds between C8 or pseudo-triloop nucleotides and IRP1 or through their impact on an induced fit mechanism of binding.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.