ArticleJournal of neurophysiology2010
Ca2+ dependence of the binomial parameters p and n at the mouse neuromuscular junction.
Article in Journal of neurophysiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Exogenous nanomolar zinc ion (ZnBiometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2025Article
- Why is binding of a divalent metal cation to a structural motif containing four carboxylate residues not accompanied by a conformational change?Protein science : a publication of the Protein Society · 2024Article
- The Mechanism of α2 adrenoreceptor-dependent Modulation of Neurotransmitter Release at the Neuromuscular Junctions.Neurochemical research · 2024Article
- Simulations of active zone structure and function at mammalian NMJs predict that loss of calcium channels alone is not sufficient to replicate LEMS effects.Journal of neurophysiology · 2023Article
- Presynaptic Acetylcholine Receptors Modulate the Time Course of Action Potential-Evoked Acetylcholine Quanta Secretion at Neuromuscular Junctions.Biomedicines · 2022Review
- Homeostatic Plasticity of the Mammalian Neuromuscular Junction.Advances in neurobiology · 2022Article
- The neuromuscular junction is a focal point of mTORC1 signaling in sarcopenia.Nature communications · 2020Article
- Depressed neuromuscular transmission causes weakness in mice lacking BK potassium channels.The Journal of general physiology · 2020Article
- Zebrafish neuromuscular junction: The power of N.Neuroscience letters · 2019Review
- Presynaptic mechanisms controlling calcium-triggered transmitter release at the neuromuscular junction.Current opinion in physiology · 2018Article
- Transmitter release site organization can predict synaptic function at the neuromuscular junction.Journal of neurophysiology · 2018Article
- Presynaptic active zones of mammalian neuromuscular junctions: Nanoarchitecture and selective impairments in aging.Neuroscience research · 2018Review
- Homeostatic synaptic plasticity at the neuromuscular junction in myasthenia gravis.Annals of the New York Academy of Sciences · 2018Review
- Depressed Synaptic Transmission and Reduced Vesicle Release Sites in Huntington's Disease Neuromuscular Junctions.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Modelling the response to low-frequency repetitive nerve stimulation of myasthenia gravis and Lambert-Eaton myasthenic syndrome.Medical & biological engineering & computing · 2016Article
- Reversible Recruitment of a Homeostatic Reserve Pool of Synaptic Vesicles Underlies Rapid Homeostatic Plasticity of Quantal Content.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Sodium channel slow inactivation as a therapeutic target for myotonia congenita.Annals of neurology · 2015Article
- Organization and function of transmitter release sites at the neuromuscular junction.The Journal of physiology · 2013Review
- Structural and functional maturation of active zones in large synapses.Molecular neurobiology · 2013Review
- Skeletal muscle IP3R1 receptors amplify physiological and pathological synaptic calcium signals.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The Ca(2+) dependence of synaptic quantal release is generally thought to be restricted to probability of vesicular release. However, some studies have suggested that the number of release sites (n) at the neuromuscular junction (NMJ) is also Ca(2+) dependent. In this study, we recorded endplate currents over a wide range of extracellular Ca(2+) concentrations and found the expected Ca(2+) dependency of release. A graphical technique was used to estimate p (probability of release) and n using standard binomial assumptions. The results suggested n was Ca(2+) dependent. The data were simulated using compound binomial statistics with variable n (Ca(2+) dependent) or fixed n (Ca(2+) independent). With fixed n, successful simulation of increasing Ca(2+) required that p increase abruptly at some sites from very low to high values. Successful simulation with variable n required the introduction of previously silent release sites (p = 0) with high values of p. Thus the success of both simulations required abrupt, large increases of p at a subset of release sites with initially low or zero p. Estimates of the time course of release obtained by deconvolving evoked endplate currents with average miniature endplate currents decreased slightly as Ca(2+) increased, thus arguing against sequential release of multiple quanta at higher Ca(2+) levels. Our results suggest that the apparent Ca(2+) dependence of n at the NMJ can be explained by an underlying Ca(2+) dependence of a spatially variable p such that p increases abruptly at a subset of sites as Ca(2+) is increased.
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