Evidence map›Paper›PMID 19939937›Full record

ArticleNucleic acids research2010

The hydrophobic patch of ubiquitin is required to protect transactivator-promoter complexes from destabilization by the proteasomal ATPases.

Chase T Archer, Thomas Kodadek

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.8field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 10 citations in OpenAlex.

  1. Review
  2. Regulation of gene expression by the ubiquitin-proteasome system.Seminars in cell & developmental biology · 2012
    Review
  3. Article
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Chase T ArcherDepartment of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390-9185, USA.
Thomas Kodadek
The University of Texas Southwestern Medical Center · US

Funding

Chemical Inhibitors of the Proteasomal ATPasesR01GM087283 · NIGMS · SCRIPPS FLORIDA · PI KODADEK, THOMAS J. · 2009 to 2010
$755k
NIGMS NIH HHS GM 087283NIGMS NIH HHS R01 GM087283
6 · The paper itself

Abstract

Mono-ubiquitylation of a transactivator is known to promote transcriptional activation of certain transactivator proteins. For the Sacchromyces cerevisiae transactivator, GAL4, attachment of mono-ubiquitin prevents destabilization of the DNA-transactivator complex by the ATPases of the 26S proteasome. This inhibition of destabilization depends on the arrangement of ubiquitin; a chain of ubiquitin tetramers linked through lysine 48 did not display the same protective effect as mono-ubiquitin. This led to an investigation into the properties of ubiquitin that may be responsible for this difference in activity between the different forms. We demonstrate the ubiquitin tetramers linked through lysine 63 do protect from proteasomal-mediated destabilization. In addition, we show that the mutating the isoleucine residue at position 44 interferes with proteasomal interaction in vitro and will abolish the protective activity in vivo. Together, these data implicate the hydrophobic patch of ubiquitin as required to protect transactivators from destabilization by the proteasomal ATPases.

Indexed as

Adenosine TriphosphatasesDNA-Binding ProteinsHydrophobic and Hydrophilic InteractionsIsoleucineMutationPromoter Regions, GeneticProteasome Endopeptidase ComplexSaccharomyces cerevisiae ProteinsTrans-ActivatorsTranscription FactorsUbiquitinAdenosine TriphosphatasesDNA-Binding ProteinsGAL4 protein, S cerevisiaeIsoleucineProteasome Endopeptidase ComplexRPT1 protein, S cerevisiaeSaccharomyces cerevisiae ProteinsTrans-ActivatorsTranscription FactorsUbiquitin

Identifiers

PMID19939937
PMCPMC2817475
OpenAlexW2093693973

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.