ArticleJournal of translational medicine2009
Let-7 microRNAs are developmentally regulated in circulating human erythroid cells.
Article in Journal of translational medicine, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
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Who cites it
36 citing papers in PubMed, 56 citations in OpenAlex.
- Expression Profile and Role of the IGF2BP1-3 Genes During Human in vitro Osteogenic Differentiation.Cell biochemistry and biophysics · 2026Article
- Small RNAs, big impact: microRNAs in malaria pathogenesis, therapeutic opportunities, and challenges.Frontiers in cellular and infection microbiology · 2026Review
- Upregulation of miR-17-3p is associated with HbF in patients with β-thalassemia and induces γ-globin expression by targeting BCL11A.Orphanet journal of rare diseases · 2025Article
- Decoding HbF reactivation by hydroxyurea in hemoglobinopathy patients through microRNA signatures.Annals of hematology · 2025Article
- Regulation of HSC development and function by Lin28b.Frontiers in cell and developmental biology · 2025Review
- Multi-omics in HIV: searching insights to understand immunological non-response in PLHIV.Frontiers in immunology · 2023Review
- MicroRNAs miR-451a and Let-7i-5p Profiles in Circulating Exosomes Vary among Individuals with Different Sickle Hemoglobin Genotypes and Malaria.Journal of clinical medicine · 2022Article
- MiR-451a and let-7i-5p loaded extracellular vesicles attenuate heme-induced inflammation in hiPSC-derived endothelial cells.Frontiers in immunology · 2022Article
- The Role of miRNAs as Therapeutic Tools in Sickle Cell Disease.Medicina (Kaunas, Lithuania) · 2021Review
- Epigenetic effects toward new insights as potential therapeutic target in B-thalassemia.Journal, genetic engineering & biotechnology · 2021Article
- Distinct miRNA Signatures and Networks Discern Fetal from Adult Erythroid Differentiation and Primary from Immortalized Erythroid Cells.International journal of molecular sciences · 2021Article
- Circulating miR-216a as a biomarker of metabolic alterations and obesity in women.Non-coding RNA research · 2020Article
- 14q32 and let-7 microRNAs regulate transcriptional networks in fetal and adult human erythroblasts.Human molecular genetics · 2018Article
- Tough decoy targeting of predominant let-7 miRNA species in adult human hematopoietic cells.Journal of translational medicine · 2017Article
- Article
- IGF2BP1 overexpression causes fetal-like hemoglobin expression patterns in cultured human adult erythroblasts.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Differentially expressed miRNAs in oxygen‑induced retinopathy newborn mouse models.Molecular medicine reports · 2017Article
- Developmental regulation of myeloerythroid progenitor function by the Lin28b-let-7-Hmga2 axis.The Journal of experimental medicine · 2016Article
- Engineering Hematopoietic Stem Cells: Lessons from Development.Cell stem cell · 2016Review
- MicroRNA Expression in β-Thalassemia and Sickle Cell Disease: A Role in The Induction of Fetal Hemoglobin.Cell journal · 2016Review
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 2 countries.
Funding
Abstract
backgroundMicroRNAs are approximately 22nt-long small non-coding RNAs that negatively regulate protein expression through mRNA degradation or translational repression in eukaryotic cells. Based upon their importance in regulating development and terminal differentiation in model systems, erythrocyte microRNA profiles were examined at birth and in adults to determine if changes in their abundance coincide with the developmental phenomenon of hemoglobin switching.
methodsExpression profiling of microRNA was performed using total RNA from four adult peripheral blood samples compared to four cord blood samples after depletion of plasma, platelets, and nucleated cells. Labeled RNAs were hybridized to custom spotted arrays containing 474 human microRNA species (miRBase release 9.1). Total RNA from Epstein-Barr virus (EBV)-transformed lymphoblastoid cell lines provided a hybridization reference for all samples to generate microRNA abundance profile for each sample.
resultsAmong 206 detected miRNAs, 79% of the microRNAs were present at equivalent levels in both cord and adult cells. By comparison, 37 microRNAs were up-regulated and 4 microRNAs were down-regulated in adult erythroid cells (fold change > 2; p < 0.01). Among the up-regulated subset, the let-7 miRNA family consistently demonstrated increased abundance in the adult samples by array-based analyses that were confirmed by quantitative PCR (4.5 to 18.4 fold increases in 6 of 8 let-7 miRNA). Profiling studies of messenger RNA (mRNA) in these cells additionally demonstrated down-regulation of ten let-7 target genes in the adult cells.
conclusionThese data suggest that a consistent pattern of up-regulation among let-7 miRNA in circulating erythroid cells occurs in association with hemoglobin switching during the fetal-to-adult developmental transition in humans.
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Registered trials
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