ArticleSystems biology in reproductive medicine2009
A novel nuclease activity that is activated by Ca(2+) chelated to EGTA.
Article in Systems biology in reproductive medicine, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 30 citations in OpenAlex.
- Article
- Article
- Review
- The mouse epididymal amyloid matrix is a mammalian counterpart of a bacterial biofilm.iScience · 2024Article
- Stealthy Player in Lipid Experiments? EDTA Binding to Phosphatidylcholine Membranes Probed by Simulations and Monolayer Experiments.The journal of physical chemistry. B · 2023Article
- Applying EDTA in Chelating Excess Metal Ions to Improve Downstream DNA Recovery from Mine Tailings for Long-Read Amplicon Sequencing of Acidophilic Fungi Communities.Journal of fungi (Basel, Switzerland) · 2022Article
- Bacterial non-specific nucleases of the phospholipase D superfamily and their biotechnological potential.Applied microbiology and biotechnology · 2020Review
- Ultraspecific analyte detection by direct kinetic fingerprinting of single molecules.Trends in analytical chemistry : TRAC · 2020Article
- Fatherhood and Sperm DNA Damage in Testicular Cancer Patients.Frontiers in endocrinology · 2018Review
- Luminal fluid of epididymis and vas deferens contributes to sperm chromatin fragmentation.Human reproduction (Oxford, England) · 2015Article
- WT1 Expression in Circulating RNA as a Minimal Residual Disease Marker for AML Patients After Stem-Cell Transplantation.Molecular diagnosis & therapy · 2015Article
- Article
- Deficient tryptophan catabolism along the kynurenine pathway reveals that the epididymis is in a unique tolerogenic state.The Journal of biological chemistry · 2011Article
- Mammalian sperm chromatin as a model for chromatin function in DNA degradation and DNA replication.Systems biology in reproductive medicine · 2011Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Most nucleases require a divalent cation as a cofactor, usually Mg(2+) or Ca(2+), and are inhibited by the chelators EDTA and EGTA. We report the existence of a novel nuclease activity, initially identified in the luminal fluids of the mouse male reproductive tract but subsequently found in other tissues,that requires EGTA chelated to calcium to digest DNA. We refer to this unique enzyme as CEAN (Chelated EGTA Activated Nuclease). Using a fraction of vas deferens luminal fluid, plasmid DNA was degraded in the presence of excess Ca(2+) (Ca(2+) :EGTA = 16) or excess EGTA (Ca(2+) :EGTA = 0.25), but required the presence of both. Higher levels of EGTA (Ca(2+) :EGTA = 0.10) prevented activity, suggesting that unchelated EGTA may be a competitive inhibitor. The EGTA-Ca(2+) activation of CEAN is reversible as removing EGTA-Ca(2+) stops ongoing DNA degradation, but adding EGTA-Ca(2+) again reactivates the enzyme. This suggests the possibility that CEAN binds directly to EGTA-Ca(2+). CEAN has a greater specificity for the chelator than for the divalent cation. Two other chelators, BAPTA and sodium citrate, do not activate CEAN in the presence of cation, but chelated EDTA does. EGTA chelated to other divalent cations such as Mn(2+), Zn(2+) , and Cu(2+) activate CEAN, but not Mg(2+) . The activity is lost upon boiling suggesting that it is a protein. These data suggest that EGTA and EDTA may not always protect DNA from nuclease damage.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.