ArticleThe Journal of cell biology1991
Low viscosity in the aqueous domain of cell cytoplasm measured by picosecond polarization microfluorimetry.
Article in The Journal of cell biology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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Who cites it
65 citing papers in PubMed, 254 citations in OpenAlex.
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- Review
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- Hydrogels as functional components in artificial cell systems.Nature reviews. Chemistry · 2022Review
- The Thermodynamics of Medial Vascular Calcification.Frontiers in cell and developmental biology · 2021Review
- Challenges for biophysical modeling of microstructure.Journal of neuroscience methods · 2020Review
- Quantifying Temperature Compensation of Bicoid Gradients with a Fast T-Tunable Microfluidic Device.Biophysical journal · 2020Article
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- Back to the Future: Genetically Encoded Fluorescent Proteins as Inert Tracers of the Intracellular Environment.International journal of molecular sciences · 2020Review
- A dynamical model of oncotripsy by mechanical cell fatigue: selective cancer cell ablation by low-intensity pulsed ultrasound.Proceedings. Mathematical, physical, and engineering sciences · 2020Article
- Potential Hydrodynamic Cytoplasmic Transfer between Mammalian Cells: Cell-Projection Pumping.Biophysical journal · 2020Article
- Single-molecule optical microscopy of protein dynamics and computational analysis of images to determine cell structure development in differentiatingComputational and structural biotechnology journal · 2020Article
- Quantitative fluorescence imaging determines the absolute number of locked nucleic acid oligonucleotides needed for suppression of target gene expression.Nucleic acids research · 2019Article
- Non-specific interactions govern cytosolic diffusion of nanosized objects in mammalian cells.Nature materials · 2018Article
- Non-invasive Neurite Mechanics in Differentiated PC12 Cells.Frontiers in cellular neuroscience · 2018Article
- Brain Metabolite Diffusion from Ultra-Short to Ultra-Long Time Scales: What Do We Learn, Where Should We Go?Frontiers in neuroscience · 2018Article
- Picosecond orientational dynamics of water in living cells.Nature communications · 2017Article
5 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Information about the rheological characteristics of the aqueous cytoplasm can be provided by analysis of the rotational motion of small polar molecules introduced into the cell. To determine fluid-phase cytoplasmic viscosity in intact cells, a polarization microscope was constructed for measurement of picosecond anisotropy decay of fluorescent probes in the cell cytoplasm. We found that the rotational correlation time (tc) of the probes, 2,7-bis-(2-carboxyethyl)-5-(and-6-)carboxyfluorescein (BCECF), 6-carboxyfluorescein, and 8-hydroxypyrene-1,3,6-trisulfonic acid (HPTS) provided a direct measure of fluid-phase cytoplasmic viscosity that was independent of probe binding. In quiescent Swiss 3T3 fibroblasts, tc values were 20-40% longer than those in water, indicating that the fluid-phase cytoplasm is only 1.2-1.4 times as viscous as water. The activation energy of fluid-phase cytoplasmic viscosity was 4 kcal/mol, which is similar to that of water. Fluid-phase cytoplasmic viscosity was altered by less than 10% upon addition of sucrose to decrease cell volume, cytochalasin B to disrupt cell cytoskeleton, and vasopressin to activate phospholipase C. Nucleoplasmic and peripheral cytoplasmic viscosities were not different. Our results establish a novel method to measure fluid-phase cytoplasmic viscosity, and indicate that fluid-phase cytoplasmic viscosity in fibroblasts is similar to that of free water.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.