Evidence map›Paper›PMID 19936989›Full record

ArticleJournal of mammary gland biology and neoplasia2009

Methods for preparing fluorescent and neutral red-stained whole mounts of mouse mammary glands.

John D Landua, Adriana P Visbal, Michael T Lewis

Open access · greenAbstract read
In one paragraph

Article in Journal of mammary gland biology and neoplasia, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
0.7field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 22 citations in OpenAlex.

  1. Review
  2. Article
  3. Journal of cell science · 2017
    Article
  4. Epithelial and non-epithelialDevelopment (Cambridge, England) · 2017
    Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Subgross breast pathology in the twenty-first century.Virchows Archiv : an international journal of pathology · 2012
    Article
  14. Article
  15. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

John D LanduaThe Lester and Sue Smith Breast Center and Department of Molecular and Cellular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA.
Adriana P Visbal
Michael T Lewis
Baylor College of Medicine · US

Funding

TRANSFORMING GROWTH FACTOR ALPHA IN PATHOGENESIS OF BREAST CANCERP01CA030195 · NCI · UNIVERSITY OF TEXAS HLTH SCI CTR SAN ANT · PI OESTERREICH, STEFFI · 1985 to 2009
$16.8M
The functional role of microRNAs in the normal mammary glandP01HD038129 · NICHD · UNIVERSITY OF COLORADO DENVER · PI ANDERSON, STEVEN M · 2000 to 2015
$15.2M
Regulation of Mammary Stem CellsR01CA127857 · NCI · BAYLOR COLLEGE OF MEDICINE · PI LEWIS, MICHAEL T. · 2008 to 2012
$1.7M
NCI NIH HHS P01 CA030195NCI NIH HHS R01 CA127857NICHD NIH HHS P01 HD038129
6 · The paper itself

Abstract

Whole mount preparations of mouse mammary glands are useful for evaluating overall changes in growth and morphology, and are essential for detecting and evaluating focal or regionally-localized phenotypes that would be difficult to detect or analyze using other techniques. We present three newly developed methods for preparing whole mounts of mammary glands from genetically-engineered mice expressing fluorescent proteins, as well as using either neutral red or a variety of fluorescent dyes. Unlike traditional hematoxylin- or carmine-stained preparations, neutral red-stained and some fluorescent preparations can be used for several common downstream analyses.

Indexed as

AnimalsFemaleFluorescent DyesMammary Glands, AnimalMiceMice, TransgenicMicroscopy, FluorescenceNeutral RedStaining and LabelingTissue and Organ HarvestingFluorescent DyesNeutral Red

Identifiers

PMID19936989
PMCPMC3038127
OpenAlexW2134065236

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.