ArticleJournal of industrial microbiology & biotechnology2010
A rapid method for the detection of representative coliforms in water samples: polymerase chain reaction-enzyme-linked immunosorbent assay (PCR-ELISA).
Article in Journal of industrial microbiology & biotechnology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Recent developments in waterborne pathogen detection technologies.Environmental monitoring and assessment · 2025Review
- Nucleic acid lateral flow assay for simultaneous detection of hygiene indicator bacteria.Analytical and bioanalytical chemistry · 2021Article
- Development of Fluorescence In Situ Hybridization as a Rapid, Accurate Method for Detecting Coliforms in Water Samples.Biosensors · 2020Article
- Rapid detection of coliforms in drinking water of Arak city using multiplex PCR method in comparison with the standard method of culture (Most Probably Number).Asian Pacific journal of tropical biomedicine · 2014Article
- Application of PCR-ELISA in molecular diagnosis.BioMed research international · 2014Review
- Development of SCAR primers based on a repetitive DNA fingerprint for Escherichia coli detection.Journal of microbiology (Seoul, Korea) · 2013Article
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Authors and funding
5 authors.
Funding
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Abstract
Methods to detect the presence of coliform bacteria in drinking water usually involve a series of complex cultivating steps that are time-consuming and subject to external influences. For this reason, the new 16S rRNA probe has been developed in this study as an alternative detector PCR-ELISA technique that does not involve the culture of bacteria and that is able to detect, identify, and quantify the representative coliform species present in water samples. Our results indicate that this technique is both rapid (detection time of 4 h) and accurate (1.4% error rate). The limit of detection (LOD) was 5 CFU/100 ml for total coliforms, which meets the standards set by most countries for drinking water. Our comparative study demonstrated that this PCR-ELISA method is superior to current conventional methods in terms of detection time, LOD, and accuracy.
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Registered trials
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