ArticleJournal of fish biology2008
Construction of a robust microarray from a non-model species (largemouth bass) using pyrosequencing technology.
Article in Journal of fish biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed.
- Fish in focus: Navigating organ damage assessment through analytical avenues - A comprehensive review.Toxicology reports · 2024Review
- Article
- Ecotoxico-lipidomics: An emerging concept to understand chemical-metabolic relationships in comparative fish models.Comparative biochemistry and physiology. Part D, Genomics & proteomics · 2020Review
- Tissue-Based Mapping of the Fathead Minnow (Frontiers in endocrinology · 2018Article
- Ecotoxicogenomic approaches for understanding molecular mechanisms of environmental chemical toxicity using aquatic invertebrate, Daphnia model organism.International journal of molecular sciences · 2015Review
- Transcriptome analysis gene expression in the liver of Coilia nasus during the stress response.BMC genomics · 2014Article
- Methylmercury-induced changes in gene transcription associated with neuroendocrine disruption in largemouth bass (Micropterus salmoides).General and comparative endocrinology · 2014Review
- Gene expression networks underlying ovarian development in wild largemouth bass (Micropterus salmoides).PloS one · 2013Article
- Genomic approaches to study genetic and environmental influences on fish sex determination and differentiation.Marine biotechnology (New York, N.Y.) · 2012Review
- Article
- Applications for next-generation sequencing in fish ecotoxicogenomics.Frontiers in genetics · 2012Article
- Cloning and expression of the translocator protein (18 kDa), voltage-dependent anion channel, and diazepam binding inhibitor in the gonad of largemouth bass (Micropterus salmoides) across the reproductive cycle.General and comparative endocrinology · 2011Article
- Applications of next generation sequencing in molecular ecology of non-model organisms.Heredity · 2011Review
- Global analysis of gene expression in mineralizing fish vertebra-derived cell lines: new insights into anti-mineralogenic effect of vanadate.BMC genomics · 2011Article
- Methoxychlor affects multiple hormone signaling pathways in the largemouth bass (Micropterus salmoides) liver.Aquatic toxicology (Amsterdam, Netherlands) · 2011Article
- The maternal and early embryonic transcriptome of the milkweed bug Oncopeltus fasciatus.BMC genomics · 2011Article
- Characterization of the rainbow trout transcriptome using Sanger and 454-pyrosequencing approaches.BMC genomics · 2010Article
- Queen conch (Strombus gigas) testis regresses during the reproductive season at nearshore sites in the Florida Keys.PloS one · 2010Article
- Genomic and proteomic responses to environmentally relevant exposures to dieldrin: indicators of neurodegeneration?Toxicological sciences : an official journal of the Society of Toxicology · 2010Article
- Effects of acute dieldrin exposure on neurotransmitters and global gene transcription in largemouth bass (Micropterus salmoides) hypothalamus.Neurotoxicology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
A novel custom microarray for largemouth bass (Micropterus salmoides) was designed with sequences obtained from a normalized cDNA library using the 454 Life Sciences GS-20 pyrosequencer. This approach yielded in excess of 58 million bases of high-quality sequence. The sequence information was combined with 2,616 reads obtained by traditional suppressive subtractive hybridizations to derive a total of 31,391 unique sequences. Annotation and coding sequences were predicted for these transcripts where possible. 16,350 annotated transcripts were selected as target sequences for the design of the custom largemouth bass oligonucleotide microarray. The microarray was validated by examining the transcriptomic response in male largemouth bass exposed to 17beta-oestradiol. Transcriptomic responses were assessed in liver and gonad, and indicated gene expression profiles typical of exposure to oestradiol. The results demonstrate the potential to rapidly create the tools necessary to assess large scale transcriptional responses in non-model species, paving the way for expanded impact of toxicogenomics in ecotoxicology.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.