ArticleMolecular vision2009
The zebrafish lens proteome during development and aging.
Article in Molecular vision, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
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Who cites it
36 citing papers in PubMed, 52 citations in OpenAlex.
- Toxicoproteomic analysis reveals arsenic-induced alterations in eye lens proteins of Labeo rohita.Fish physiology and biochemistry · 2026Article
- Contributions of aquatic organisms to understanding the vertebrate ocular lens.Frontiers in ophthalmology · 2026Review
- Data-Independent Acquisition Parallel Accumulation-Serial Fragmentation (diaPASEF) Analysis of the Separated Zebrafish Lens Improves Identifications.Journal of the American Society for Mass Spectrometry · 2025Article
- Transcriptomic Profiling of Zebrafish Mutant forInternational journal of molecular sciences · 2025Article
- Comparative analysis of the structure and crystallin composition of the lenses of freshwater fish and gastropods with respect to their vision.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2025Article
- Loss of αBa-crystallin, but not αA-crystallin, increases age-related cataract in the zebrafish lens.Experimental eye research · 2024Article
- Loss of αBa-crystallin, but not αA-crystallin, increases age-related cataract in the zebrafish lens.bioRxiv : the preprint server for biology · 2024Article
- The generation and characterization of a transgenic zebrafish line with lens-specific Cre expression.Molecular vision · 2024Article
- Impact of α-crystallin protein loss on zebrafish lens development.Experimental eye research · 2023Article
- Allele-specific gene expression can underlie altered transcript abundance in zebrafish mutants.eLife · 2022Article
- Generation and characterization of a novelFrontiers in cell and developmental biology · 2022Article
- Some Aspects of Development and Histological Structure of the Visual System of Nothobranchius Guentheri.Animals : an open access journal from MDPI · 2021Article
- Zebrafish Model in Ophthalmology to Study Disease Mechanism and Drug Discovery.Pharmaceuticals (Basel, Switzerland) · 2021Review
- Single cell transcriptomics of the developing zebrafish lens and identification of putative controllers of lens development.Experimental eye research · 2021Article
- Feed Restriction Modulates Growth, Gut Morphology and Gene Expression in Zebrafish.International journal of molecular sciences · 2021Article
- LIN28B affects gene expression at the hypothalamic-pituitary axis and serum testosterone levels.Scientific reports · 2019Article
- Quantifying protein dynamics and stability in a living organism.Nature communications · 2019Article
- Proteome-transcriptome analysis and proteome remodeling in mouse lens epithelium and fibers.Experimental eye research · 2019Article
- Why does the zebrafish cloche mutant develop lens cataract?PloS one · 2019Article
- The occhiolino (occ) mutant Zebrafish, a model for development of the optical function in the biological lens.Developmental dynamics : an official publication of the American Association of Anatomists · 2017Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
purposeChanges in lens protein expression during zebrafish development results in a smooth gradient of refractive index necessary for excellent optical function. Age-related changes in crystallin expression have been well documented in mammals but are poorly understood in the zebrafish.
methodsIn the zebrafish lens, a systematic analysis of protein content with age was performed using size exclusion chromatography (SEC) combined with linear trap quadrupole Fourier transform tandem mass spectrometry (LTQ-FT LC-MS/MS; rank-order shotgun) proteomics in lenses of larval, juvenile, and adult zebrafish.
resultsalpha-Crystallins, previously shown to have low abundance in the zebrafish lens, were found to increase dramatically with maturation and aging. SEC determined that beta-crystallin was predominant at 4.5 days. With age, the alpha- and gamma-crystallins increased, and a high molecular weight fraction appeared between six weeks and six months to become the dominant component by 2.5 years. Similarly, shotgun proteomics determined that beta-crystallins were the predominant proteins in the young lens. With age, the proportion of alpha- and gamma-crystallins increased dramatically. After crystallins, calpain 3, membrane, and cytoskeletal proteins were most abundant. Five new beta-crystallins and 13 new gamma-crystallins were identified.
conclusionsAs expected, SEC and proteomics demonstrated changing levels of protein expression with age, especially among the crystallins. The results also confirmed the existence of novel crystallins in the zebrafish genome.
Indexed as
Identifiers
19936306PMC2779061W2102234720What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.