ArticlePLoS neglected tropical diseases2009
An ex-vivo human intestinal model to study Entamoeba histolytica pathogenesis.
Article in PLoS neglected tropical diseases, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 136 citations in OpenAlex.
- Host-parasite interactions duringFrontiers in cellular and infection microbiology · 2026Article
- Enteropathogenic Escherichia coli induces Entamoeba histolytica superdiffusion movement on fibronectin by reducing traction forces.PLoS pathogens · 2025Article
- Work with me here: variations in genome content and emerging genetic tools in Entamoeba histolytica.Trends in parasitology · 2025Review
- Article
- Pathogenicity and virulence ofVirulence · 2023Review
- Review
- 4D live imaging and computational modeling of a functional gut-on-a-chip evaluate how peristalsis facilitates enteric pathogen invasion.Science advances · 2022Article
- The IL-33-ILC2 pathway protects from amebic colitis.Mucosal immunology · 2022Article
- Host Protective Mechanisms to Intestinal Amebiasis.Trends in parasitology · 2021Review
- Taxon-Specific Proteins of the PathogenicFrontiers in cellular and infection microbiology · 2021Review
- Article
- Whole genome sequencing of Entamoeba nuttalli reveals mammalian host-related molecular signatures and a novel octapeptide-repeat surface protein.PLoS neglected tropical diseases · 2019Article
- Curcumin Attenuates the Pathogenicity ofPathogens (Basel, Switzerland) · 2019Article
- Making sense of gut feelings in the traumatic brain injury pathogenesis.Neuroscience and biobehavioral reviews · 2019Review
- Development of a Novel Ex-vivo 3D Model to Screen Amoebicidal Activity on Infected Tissue.Scientific reports · 2019Article
- In vitro and ex vivo systems at the forefront of infection modeling and drug discovery.Biomaterials · 2019Review
- Genetic Diversity and Gene Family Expansions in Members of the Genus Entamoeba.Genome biology and evolution · 2019Article
- An ex vivo ruminal ovine model to study the immediate immune response in the context of bacterial lipopolysaccharide.Functional & integrative genomics · 2018Article
- AIG1 affects in vitro and in vivo virulence in clinical isolates of Entamoeba histolytica.PLoS pathogens · 2018Article
- Article
Corrections and comments
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Authors and funding
13 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Amoebiasis (a human intestinal infection affecting 50 million people every year) is caused by the protozoan parasite Entamoeba histolytica. To study the molecular mechanisms underlying human colon invasion by E. histolytica, we have set up an ex vivo human colon model to study the early steps in amoebiasis. Using scanning electron microscopy and histological analyses, we have established that E. histolytica caused the removal of the protective mucus coat during the first two hours of incubation, detached the enterocytes, and then penetrated into the lamina propria by following the crypts of Lieberkühn. Significant cell lysis (determined by the release of lactodehydrogenase) and inflammation (marked by the secretion of pro-inflammatory molecules such as interleukin 1 beta, interferon gamma, interleukin 6, interleukin 8 and tumour necrosis factor) were detected after four hours of incubation. Entamoeba dispar (a closely related non-pathogenic amoeba that also colonizes the human colon) was unable to invade colonic mucosa, lyse cells or induce an inflammatory response. We also examined the behaviour of trophozoites in which genes coding for known virulent factors (such as amoebapores, the Gal/GalNAc lectin and the cysteine protease 5 (CP-A5), which have major roles in cell death, adhesion (to target cells or mucus) and mucus degradation, respectively) were silenced, together with the corresponding tissue responses. Our data revealed that the signalling via the heavy chain Hgl2 or via the light chain Lgl1 of the Gal/GalNAc lectin is not essential to penetrate the human colonic mucosa. In addition, our study demonstrates that E. histolytica silenced for CP-A5 does not penetrate the colonic lamina propria and does not induce the host's pro-inflammatory cytokine secretion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.