ArticlePLoS pathogens2009
A novel signaling network essential for regulating Pseudomonas aeruginosa biofilm development.
Article in PLoS pathogens, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 125 papers.
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Who cites it
125 citing papers in PubMed, 213 citations in OpenAlex.
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- Lives in a gram of soil: linking life cycle biology to soil ecology.The ISME journal · 2026Review
- Roles of GacSA and DJ41_1407 inInternational journal of molecular sciences · 2025Article
- Microbial responses to antibiotics cryptically shift the direction of disease outcomes.Scientific reports · 2025Article
- FleQ finetunes the expression of a subset of BrlR-activated genes to enable antibiotic tolerance byJournal of bacteriology · 2025Article
- Time-resolved compositional and dynamics analysis of biofilm maturation and dispersal via solid-state NMR spectroscopy.NPJ biofilms and microbiomes · 2025Article
- Harnessing hypoxia: bacterial adaptation and chronic infection in cystic fibrosis.FEMS microbiology reviews · 2025Review
- Bacterial Adhesion to Natural and Synthetic Fibre-Forming Polymers: Influence of Material Properties.Polymers · 2024Review
- Mycobacterial biofilms: A therapeutic target against bacterial persistence and generation of antibiotic resistance.Heliyon · 2024Review
- Practical Guidance for Clinical Microbiology Laboratories: Microbiologic diagnosis of implant-associated infections.Clinical microbiology reviews · 2024Review
- Flip the switch: the role of FleQ in modulating the transition between the free-living and sessile mode of growth inJournal of bacteriology · 2024Review
- Quantifying the Dynamics of Bacterial Biofilm Formation on the Surface of Soft Contact Lens Materials Using Digital Holographic Tomography to Advance Biofilm Research.International journal of molecular sciences · 2024Article
- FlhF affects the subcellular clustering of WspR through HsbR inApplied and environmental microbiology · 2024Article
- Insights into Kinases of ESKAPE Pathogens for Therapeutic Interventions.Cardiovascular & hematological agents in medicinal chemistry · 2024Review
- Development and Regulation of the Extreme Biofilm Formation ofInternational journal of molecular sciences · 2023Article
- Manganese Acts as an Environmental Inhibitor of Pseudomonas aeruginosa Biofilm Development by Inducing Dispersion and Modulating c-di-GMP and Exopolysaccharide Production via RbdA.Journal of bacteriology · 2023Article
- Genetic and environmental determinants of surface adaptations inMicrobiology (Reading, England) · 2023Article
65 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The important human pathogen Pseudomonas aeruginosa has been linked to numerous biofilm-related chronic infections. Here, we demonstrate that biofilm formation following the transition to the surface attached lifestyle is regulated by three previously undescribed two-component systems: BfiSR (PA4196-4197) harboring an RpoD-like domain, an OmpR-like BfmSR (PA4101-4102), and MifSR (PA5511-5512) belonging to the family of NtrC-like transcriptional regulators. These two-component systems become sequentially phosphorylated during biofilm formation. Inactivation of bfiS, bfmR, and mifR arrested biofilm formation at the transition to the irreversible attachment, maturation-1 and -2 stages, respectively, as indicated by analyses of biofilm architecture, and protein and phosphoprotein patterns. Moreover, discontinuation of bfiS, bfmR, and mifR expression in established biofilms resulted in the collapse of biofilms to an earlier developmental stage, indicating a requirement for these regulatory systems for the development and maintenance of normal biofilm architecture. Interestingly, inactivation did not affect planktonic growth, motility, polysaccharide production, or initial attachment. Further, we demonstrate the interdependency of this two-component systems network with GacS (PA0928), which was found to play a dual role in biofilm formation. This work describes a novel signal transduction network regulating committed biofilm developmental steps following attachment, in which phosphorelays and two sigma factor-dependent response regulators appear to be key components of the regulatory machinery that coordinates gene expression during P. aeruginosa biofilm development in response to environmental cues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.