ArticleScience signaling2009
Ca(2+) puffs originate from preestablished stable clusters of inositol trisphosphate receptors.
Article in Science signaling, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
What it found
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Who cites it
48 citing papers in PubMed, 83 citations in OpenAlex.
- Phosphorylation of Bok at Ser-8 blocks its ability to suppress IPCell communication and signaling : CCS · 2025Article
- Cellular mechanisms underlying pituitary adenylate cyclase activating polypeptide-stimulated secretion in the adrenal medulla.Biochemical Society transactions · 2024Review
- A PACAP-activated network for secretion requires coordination of CaMolecular biology of the cell · 2024Article
- Understanding IPCell calcium · 2023Review
- Dissociation of inositol 1,4,5-trisphosphate from IPThe Journal of biological chemistry · 2023Article
- Functional determination of calcium-binding sites required for the activation of inositol 1,4,5-trisphosphate receptors.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Termination of CaCell calcium · 2021Article
- KRAP tethers IPNature communications · 2021Article
- Modeling of Astrocyte Networks: Toward Realistic Topology and Dynamics.Frontiers in cellular neuroscience · 2021Article
- Noise analysis of cytosolic calcium image data.Cell calcium · 2020Article
- Spatial-temporal patterning of CaSeminars in cell & developmental biology · 2019Review
- Simulation of calcium signaling in fine astrocytic processes: Effect of spatial properties on spontaneous activity.PLoS computational biology · 2019Article
- Applications of FLIKA, a Python-based image processing and analysis platform, for studying local events of cellular calcium signaling.Biochimica et biophysica acta. Molecular cell research · 2019Article
- Structure and Function of IPCold Spring Harbor perspectives in biology · 2019Review
- All three IPScience signaling · 2018Article
- Disentangling astroglial physiology with a realistic cell model in silico.Nature communications · 2018Article
- All three IPJournal of cell science · 2018Article
- Dynamic CaCell calcium · 2018Article
- Article
- CaNature communications · 2017Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Intracellular calcium ion (Ca(2+)) signaling crucially depends on the clustered organization of inositol trisphosphate receptors (IP(3)Rs) in the endoplasmic reticulum (ER) membrane. These ligand-gated ion channels liberate Ca(2+) to generate local signals known as Ca(2+) puffs. We tested the hypothesis that IP(3) itself elicits rapid clustering of IP(3)Rs by using flash photolysis of caged IP(3) in conjunction with high-resolution Ca(2+) imaging to monitor the activity and localization of individual IP(3)Rs within intact mammalian cells. Our results indicate that Ca(2+) puffs arising with latencies as short as 100 to 200 ms after photorelease of IP(3) already involve at least four IP(3)R channels, and that this number does not subsequently grow. Moreover, single active IP(3)Rs show limited mobility, and stochastic simulations suggest that aggregation of IP(3)Rs at puff sites by a diffusional trapping mechanism would require many seconds. We thus conclude that puff sites represent preestablished, stable clusters of IP(3)Rs and that functional IP(3)Rs are not readily diffusible within the ER membrane.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.