ArticleExperimental biology and medicine (Maywood, N.J.)2009
Characterization of TWIK-2, a two-pore domain K+ channel, cloned from the rat middle cerebral artery.
Article in Experimental biology and medicine (Maywood, N.J.), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 16 citations in OpenAlex.
- Potassium channels in vascular smooth muscle cells.Physiological reviews · 2026Review
- Insights into the structure and modulation of human TWIK-2.Nature communications · 2026Article
- Lysosomal Potassium Channels.Handbook of experimental pharmacology · 2023Review
- The TWIK2 Potassium Efflux Channel in Macrophages Mediates NLRP3 Inflammasome-Induced Inflammation.Immunity · 2018Article
- Recombinant tandem of pore-domains in a Weakly Inward rectifying KScientific reports · 2017Article
- Sensitization of neonatal rat lumbar motoneuron by the inflammatory pain mediator bradykinin.eLife · 2015Article
- Classification of 2-pore domain potassium channels based on rectification under quasi-physiological ionic conditions.Channels (Austin, Tex.) · 2014Article
- Alterations of N-3 polyunsaturated fatty acid-activated K2P channels in hypoxia-induced pulmonary hypertension.Basic & clinical pharmacology & toxicology · 2013Article
- Protein kinase A and C regulate leak potassium currents in freshly isolated vascular myocytes from the aorta.PloS one · 2013Article
- Impairment of neurovascular coupling in type 1 diabetes mellitus in rats is linked to PKC modulation of BK(Ca) and Kir channels.American journal of physiology. Heart and circulatory physiology · 2012Article
- Two-pore domain K⁺ channels regulate membrane potential of isolated human articular chondrocytes.The Journal of physiology · 2011Article
- Disruption of K(2P)6.1 produces vascular dysfunction and hypertension in mice.Hypertension (Dallas, Tex. : 1979) · 2011Article
- Cerebrovascular responses in mice deficient in the potassium channel, TREK-1.American journal of physiology. Regulatory, integrative and comparative physiology · 2010Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
TWIK-2, a member of the Two-Pore Domain K channel family, is expressed in a number of mammalian tissues including the vascular system. The function of TWIK-2 is not known. The purpose of this study was to clone the TWIK-2 channel from the rat middle cerebral artery, express it in CHO cells, and characterize the channel's electrical properties. In light of the fact that there are no specific TWIK-2 inhibitors or activators, a better characterization of the channel should enhance our understanding of its role in the vascular system. TWIK-2 was cloned from the rat middle cerebral artery and expressed with an N-terminal green fluorescence protein (GFP) in CHO cells. We report that rTWIK-2-GFP currents were relatively linear at physiological K(+) concentrations but become slightly inwardly rectifying in symmetrical K(+). rTWIK-2-GFP was insensitive to 10 mM TEA, 3 mM 4-aminopyridine, and 10 microM glibenclamide. However, rTWIK-2-GFP was inhibited by Ba(2+) with 50% of the current being blocked at 80 microM. rTWIK-2-GFP activity was enhanced 60% by 100 microM arachidonic acid. The electrophysiological characteristics of TWIK-2 indicate that it could serve an important role in ion homeostasis and regulation of the membrane potential in arteries and arterioles.
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