ArticleExperimental biology and medicine (Maywood, N.J.)2009
Brucella melitensis, B. neotomae and B. ovis elicit common and distinctive macrophage defense transcriptional responses.
Article in Experimental biology and medicine (Maywood, N.J.), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 37 citations in OpenAlex.
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- Immunological pathways of macrophage response toInnate immunity · 2020Article
- Survival of Brucella abortus S19 and other Brucella spp. in the presence of oxidative stress and within macrophages.Folia microbiologica · 2020Article
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- Transcriptome analysis ofAccess microbiology · 2020Article
- Intracellular invasion and survival of Brucella neotomae, another possible zoonotic Brucella species.PloS one · 2019Article
- TheThe Journal of biological chemistry · 2017Article
- SOCS3 revisited: a broad regulator of disease, now ready for therapeutic use?Cellular and molecular life sciences : CMLS · 2016Review
- Draft Genome Sequences of Brucella suis Biovar 4 Strain NCTC 10385, Brucella ceti Strain NCTC 12891T, Brucella inopinata Strain CAMP 6436T, and Brucella neotomae Strain ATCC 23459T.Genome announcements · 2014Article
- Key role of Toll-like receptor 2 in the inflammatory response and major histocompatibility complex class ii downregulation in Brucella abortus-infected alveolar macrophages.Infection and immunity · 2014Article
- The predicted ABC transporter AbcEDCBA is required for type IV secretion system expression and lysosomal evasion by Brucella ovis.PloS one · 2014Article
- Brucella dissociation is essential for macrophage egress and bacterial dissemination.Frontiers in cellular and infection microbiology · 2014Article
- Early transcriptional responses of internalization defective Brucella abortus mutants in professional phagocytes, RAW 264.7.BMC genomics · 2013Article
- Transcriptome analysis of HeLa cells response to Brucella melitensis infection: a molecular approach to understand the role of the mucosal epithelium in the onset of the Brucella pathogenesis.Microbes and infection · 2012Article
- Chd2 interacts with H3.3 to determine myogenic cell fate.The EMBO journal · 2012Article
- Antigen-specific acquired immunity in human brucellosis: implications for diagnosis, prognosis, and vaccine development.Frontiers in cellular and infection microbiology · 2012Article
- Analyses of Brucella pathogenesis, host immunity, and vaccine targets using systems biology and bioinformatics.Frontiers in cellular and infection microbiology · 2012Review
- Deep-sequencing analysis of the mouse transcriptome response to infection with Brucella melitensis strains of differing virulence.PloS one · 2011Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Brucella spp. establish an intracellular replicative niche in macrophages, while macrophages attempt to eliminate the bacteria by innate defense mechanisms. Brucella spp. possess similar genomes yet exhibit different macrophage infections. Few B. melitensis and B. neotomae enter macrophages with intracellular adaptation occurring over 4-8 hr. Conversely, B. ovis are readily ingested by macrophages and exhibit a persistent plateau of infection. Evaluating early macrophage interaction with Brucella spp. allows discovery of host entry and intracellular translocation mechanisms. Microarray analysis of macrophage transcriptional response following a 4 hr infection by different Brucella spp. revealed common macrophage genes altered in expression compared to uninfected macrophages. Macrophage infection with three different Brucella spp. provokes a common innate immune theme with increased transcript levels of chemokines and defense response genes and decreased transcript levels of GTPase signaling and cytoskeletal function that may affect trafficking of Brucella containing vesicles. For example, transcript levels of genes associated with chemotaxis (IL-1beta, MIP-1alpha), cytokine regulation (Socs3) and defense (Fas, Tnf) were increased, while transcript levels of genes associated with vesicular trafficking (Rab3d) and lysosomal associated enzymes (prosaposin) were decreased. Genes with altered macrophage transcript levels among Brucella spp. infections may correlate with species specific host defenses and intracellular survival strategies. Depending on the infecting Brucella species, gene ontology categorization identified genes differentially involved in cell growth and maintenance, endopeptidase inhibitor activity and G-protein mediated signaling. Examples of decreased gene expression in B. melitensis infection but not other Brucella spp. were growth arrest (Gas2), immunoglobulin receptor (FcgammarI) and chemokine receptor (Cxcr4) genes, suggesting opposing effects on intracellular functions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.