ArticleProceedings of the National Academy of Sciences of the United States of America2009
Use of high throughput sequencing to observe genome dynamics at a single cell level.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- scMVAF: a multi-view adaptive fusion clustering approach for single-cell RNA-sequencing data.Briefings in bioinformatics · 2026Article
- Revisiting classicalMicrobial genomics · 2025Article
- Integrated Metabolomics and Transcriptomics Analyses Identify Key Amino Acid Metabolic Mechanisms inFoods (Basel, Switzerland) · 2025Article
- Exploring the Role of Clustered Mutations in Carcinogenesis and Their Potential Clinical Implications in Cancer.International journal of molecular sciences · 2024Review
- A diet of oxidative stress-adapted bacteria improves stress resistance and lifespan inScience advances · 2024Article
- Identification and characterization of non-coding RNA networks in infected macrophages revealing the pathogenesis of F. nucleatum-associated diseases.BMC genomics · 2022Article
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- Topokaryotyping demonstrates single cell variability and stress dependent variations in nuclear envelope associated domains.Nucleic acids research · 2018Article
- Real-time dynamics of mutagenesis reveal the chronology of DNA repair and damage tolerance responses in single cells.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- A streptomycin resistance marker inPeerJ · 2018Article
- A comprehensive survey of the mutagenic impact of common cancer cytotoxics.Genome biology · 2016Article
- Genomic approaches to DNA repair and mutagenesis.DNA repair · 2015Review
- Clusters of Multiple Mutations: Incidence and Molecular Mechanisms.Annual review of genetics · 2015Review
- Identifying structural variation in haploid microbial genomes from short-read resequencing data using breseq.BMC genomics · 2014Article
- Article
- Developing insights into the mechanisms of evolution of bacterial pathogens from whole-genome sequences.Future microbiology · 2012Review
- Two mechanisms produce mutation hotspots at DNA breaks in Escherichia coli.Cell reports · 2012Article
- Escherichia coli frameshift mutation rate depends on the chromosomal context but not on the GATC content near the mutation site.PloS one · 2012Article
- Damage-induced localized hypermutability.Cell cycle (Georgetown, Tex.) · 2011Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
With the development of high throughput sequencing technology, it becomes possible to directly analyze mutation distribution in a genome-wide fashion, dissociating mutation rate measurements from the traditional underlying assumptions. Here, we sequenced several genomes of Escherichia coli from colonies obtained after chemical mutagenesis and observed a strikingly nonrandom distribution of the induced mutations. These include long stretches of exclusively G to A or C to T transitions along the genome and orders of magnitude intra- and intergenomic differences in mutation density. Whereas most of these observations can be explained by the known features of enzymatic processes, the others could reflect stochasticity in the molecular processes at the single-cell level. Our results demonstrate how analysis of the molecular records left in the genomes of the descendants of an individual mutagenized cell allows for genome-scale observations of fixation and segregation of mutations, as well as recombination events, in the single genome of their progenitor.
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