ArticleOsteoarthritis and cartilage2010
Phenotypic characterization of epiphycan-deficient and epiphycan/biglycan double-deficient mice.
Article in Osteoarthritis and cartilage, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 61 citations in OpenAlex.
- Quantitative proteomic analysis of eight cartilaginous tissues reveals characteristic differences as well as similarities between subgroups.The Journal of biological chemistry · 2012Trial
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- Apolipoprotein E is a novel marker for chondrocytes in the growth plate resting zone.Research square · 2024Article
- Cartilage decellularized matrix hydrogel loaded with protocatechualdehyde for targeted epiphycan treatment of osteoarthritis.Materials today. Bio · 2024Article
- Effects of immediate loading directionality on the mechanical sensing protein PIEZO1 expression and early-stage healing process of peri-implant bone.Biomedical engineering online · 2024Article
- Osteomodulin downregulation is associated with osteoarthritis development.Bone research · 2023Article
- Unravelling the limb regeneration mechanisms of Polypedates maculatus, a sub-tropical frog, by transcriptomics.BMC genomics · 2023Article
- Review
- Benefits of lumican on human bone health: clinical evidence using bone marrow aspirates.The Korean journal of internal medicine · 2022Article
- Differentiated activities of decorin and biglycan in the progression of post-traumatic osteoarthritis.Osteoarthritis and cartilage · 2021Article
- Males and Females Have Distinct Molecular Events in the Articular Cartilage during Knee Osteoarthritis.International journal of molecular sciences · 2021Article
- Lumican Inhibits Osteoclastogenesis and Bone Resorption by Suppressing Akt Activity.International journal of molecular sciences · 2021Article
- Decorin regulates cartilage pericellular matrix micromechanobiology.Matrix biology : journal of the International Society for Matrix Biology · 2021Article
- Epiphycan Predicts Poor Outcomes and Promotes Metastasis in Ovarian Cancer.Frontiers in oncology · 2021Article
- Palaeoproteomic identification of breast milk protein residues from the archaeological skeletal remains of a neonatal dog.Scientific reports · 2019Article
- Human mesenchymal stem cells induced to differentiate as chondrocytes follow a biphasic pattern of extracellular matrix production.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2018Article
- In vivo effect of opticin deficiency in cartilage in a surgically induced mouse model of osteoarthritis.Scientific reports · 2018Article
- Proteoglycan form and function: A comprehensive nomenclature of proteoglycans.Matrix biology : journal of the International Society for Matrix Biology · 2015Review
Corrections and comments
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Authors and funding
13 authors at 4 institutions in 1 country.
Funding
Abstract
objectiveTo characterize the in vivo role epiphycan (Epn) has in cartilage development and/or maintenance.
methodsEpn-deficient mice were generated by disrupting the Epn gene in mouse embryonic stem cells. Epn/biglycan (Bgn) double-deficient mice were produced by crossing Epn-deficient mice with Bgn-deficient mice. Whole knee joint histological sections were stained using van Gieson or Fast green/Safranin-O to analyze collagen or proteoglycan content, respectively. Microarray analysis was performed to detect gene expression changes within knee joints.
resultsEpn-deficient and Epn/Bgn double-deficient mice appeared normal at birth. No significant difference in body weight or femur length was detected in any animal at 1 month of age. However, 9-month Epn/Bgn double-deficient mice were significantly lighter and had shorter femurs than wild type mice, regardless of gender. Male Epn-deficient mice also had significantly shorter femurs than wild type mice at 9 months. Most of the deficient animals developed osteoarthritis (OA) with age; the onset of OA was observed earliest in Epn/Bgn double-deficient mice. Message RNA isolated from Epn/Bgn double-deficient knee joints displayed increased matrix protein expression compared with wild type mice, including other small leucine-rich proteoglycan (SLRP) members such as asporin, fibromodulin and lumican.
conclusionSimilar to other previously studied SLRPs, EPN plays an important role in maintaining joint integrity. However, the severity of the OA phenotype in the Epn/Bgn double-deficient mouse suggests a synergy between these two proteins. These data are the first to show a genetic interaction involving class I and class III SLRPs in vivo.
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