ArticleJournal of molecular biology2010
Cation-selective pathway of OmpF porin revealed by anomalous X-ray diffraction.
Article in Journal of molecular biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Metabolic control of porin permeability influences antibiotic resistance in Escherichia coli.Nature microbiology · 2025Article
- Improved Free-Energy Estimates for the Permeation of Bulky Antibiotic Molecules through Porin Channels Using Temperature-Accelerated Sliced Sampling.Journal of chemical theory and computation · 2025Article
- Molecular Mechanism of Ciprofloxacin Translocation Through the Major Diffusion Channels of the ESKAPE PathogensThe journal of physical chemistry. B · 2024Article
- Review
- Molecular Dynamics Simulation for All.Neuron · 2018Review
- Two outer membrane proteins are bovine lactoferrin-binding proteins in Mannheimia haemolytica A1.Veterinary research · 2016Article
- Dynamics and Interactions of OmpF and LPS: Influence on Pore Accessibility and Ion Permeability.Biophysical journal · 2016Article
- A structural study of ion permeation in OmpF porin from anomalous X-ray diffraction and molecular dynamics simulations.Journal of the American Chemical Society · 2013Article
- Structural basis for ion permeation mechanism in pentameric ligand-gated ion channels.The EMBO journal · 2013Article
- The binding of antibiotics in OmpF porin.Structure (London, England : 1993) · 2013Article
- Divalent Metal Ion Transport across Large Biological Ion Channels and Their Effect on Conductance and Selectivity.Biochemistry research international · 2012Article
- Ionic partition and transport in multi-ionic channels: a molecular dynamics simulation study of the OmpF bacterial porin.Biophysical journal · 2010Article
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Authors and funding
4 authors.
Funding
Abstract
The OmpF porin from the Escherichia coli outer membrane folds into a trimer of beta-barrels, each forming a wide aqueous pore allowing the passage of ions and small solutes. A long loop (L3) carrying multiple acidic residues folds into the beta-barrel pore to form a narrow "constriction zone". A strong and highly conserved charge asymmetry is observed at the constriction zone, with multiple basic residues attached to the wall of the beta-barrel (Lys16, Arg42, Arg82 and Arg132) on one side, and multiple acidic residues of L3 (Asp107, Asp113, Glu117, Asp121, Asp126, Asp127) on the other side. Several computational studies have suggested that a strong transverse electric field could exist at the constriction zone as a result of such charge asymmetry, giving rise to separate permeation pathways for cations and anions. To examine this question, OmpF was expressed, purified and crystallized in the P6(3) space group and two different data sets were obtained at 2.6 A and 3.0 A resolution with K(+) and Rb(+), respectively. The Rb(+)-soaked crystals were collected at the rubidium anomalous wavelength of 0.8149 A and cation positions were determined. A PEG molecule was observed in the pore region for both the K(+) and Rb(+)-soaked crystals, where it interacts with loop L3. The results reveal the separate pathways of anions and cations across the constriction zone of the OmpF pore.
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