ArticleDevelopmental biology2010
Targeted deletion of the zebrafish obscurin A RhoGEF domain affects heart, skeletal muscle and brain development.
Article in Developmental biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 30 citations in OpenAlex.
- The varied functions of the giant muscle scaffold protein obscurin.Frontiers in cell and developmental biology · 2025Review
- A Rare Case of Polymicrogyria in an Elderly Individual With Unique Polygenic Underlining.Cureus · 2024Article
- Two Cases of Sporadic Amyotrophic Lateral Sclerosis With Contrasting Clinical Phenotypes: Genetic Insights.Cureus · 2024Article
- Essential role of obscurin kinase-1 in cardiomyocyte coupling via N-cadherin phosphorylation.JCI insight · 2024Article
- Abiotic and past climatic conditions drive protein abundance variation among natural populations of the caddisfly Crunoecia irrorata.Scientific reports · 2020Article
- Unraveling obscurins in heart disease.Pflugers Archiv : European journal of physiology · 2019Review
- Obscure functions: the location-function relationship of obscurins.Biophysical reviews · 2017Review
- Paxillin genes and actomyosin contractility regulate myotome morphogenesis in zebrafish.Developmental biology · 2017Article
- Dynamin Binding Protein (Tuba) Deficiency Inhibits Ciliogenesis and Nephrogenesis in Vitro and in Vivo.The Journal of biological chemistry · 2016Article
- Signatures of selection in the three-spined stickleback along a small-scale brackish water - freshwater transition zone.Ecology and evolution · 2015Article
- Mechanisms of otoconia and otolith development.Developmental dynamics : an official publication of the American Association of Anatomists · 2015Review
- Understanding cardiac sarcomere assembly with zebrafish genetics.Anatomical record (Hoboken, N.J. : 2007) · 2014Review
- Obscurins: Goliaths and Davids take over non-muscle tissues.PloS one · 2014Article
- Obscurins: unassuming giants enter the spotlight.IUBMB life · 2013Review
- Article
- Synaptic proteome changes in mouse brain regions upon auditory discrimination learning.Proteomics · 2012Article
- The function of the M-line protein obscurin in controlling the symmetry of the sarcomere in the flight muscle of Drosophila.Journal of cell science · 2012Article
- Loss of giant obscurins promotes breast epithelial cell survival through apoptotic resistance.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2012Article
- The Popeye domain containing 2 (popdc2) gene in zebrafish is required for heart and skeletal muscle development.Developmental biology · 2012Article
- Large isoforms of UNC-89 (obscurin) are required for muscle cell architecture and optimal calcium release in Caenorhabditis elegans.PloS one · 2012Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Obscurin is a giant structural and signaling protein that participates in the assembly and structural integrity of striated myofibrils. Previous work has examined the physical interactions between obscurin and other cytoskeletal elements but its in vivo role in cell signaling, including the functions of its RhoGTPase Exchange Factor (RhoGEF) domain have not been characterized. In this study, morpholino antisense oligonucleotides were used to create an in-frame deletion of the active site of the obscurin A RhoGEF domain in order to examine its functions in zebrafish development. Cardiac myocytes in the morphant embryos lacked the intercalated disks that were present in controls by 72 and, in the more severely affected embryos, the contractile filaments were not organized into mature sarcomeres. Neural abnormalities included delay or loss of retinal lamination. Rescue of the phenotype with co-injection of mini-obscurin A expression constructs demonstrated that the observed effects were due to the loss of small GTPase activation by obscurin A. The immature phenotype of the cardiac myocytes and the retinal neuroblasts observed in the morphant embryos suggests that obscurin A-mediated small GTPase signaling promotes tissue-specific cellular differentiation. This is the first demonstration of the importance of the obscurin A-mediated RhoGEF signaling in vertebrate organogenesis and highlights the central role of obscurin A in striated muscle and neural development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.