ArticleCurrent biology : CB2009
Interplay of microtubule dynamics and sliding during bipolar spindle formation in mammalian cells.
Article in Current biology : CB, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 39 citations in OpenAlex.
- Kinesin Spindle Protein (KIF11) in Mitosis and Cancer.International journal of molecular sciences · 2025Review
- Matrin3 regulates mitotic spindle dynamics by controlling alternative splicing of CDC14B.Cell reports · 2023Article
- More than two populations of microtubules comprise the dynamic mitotic spindle.Journal of cell science · 2022Article
- Review
- Small-molecule inhibition of aging-associated chromosomal instability delays cellular senescence.EMBO reports · 2020Article
- Aurora A inhibition limits centrosome clustering and promotes mitotic catastrophe in cells with supernumerary centrosomes.Oncotarget · 2019Article
- Suppressor Analysis Uncovers That MAPs and Microtubule Dynamics Balance with the Cut7/Kinesin-5 Motor for Mitotic Spindle Assembly inG3 (Bethesda, Md.) · 2019Article
- Acto-myosin force organization modulates centriole separation and PLK4 recruitment to ensure centriole fidelity.Nature communications · 2019Article
- Article
- The mitotic tensegrity guardian tau protects mammary epithelia from katanin-like1-induced aneuploidy.Oncotarget · 2016Article
- Generating a "Humanized" Drosophila S2 Cell Line Sensitive to Pharmacological Inhibition of Kinesin-5.Journal of visualized experiments : JoVE · 2016Article
- Cdk1 phosphorylates the Rac activator Tiam1 to activate centrosomal Pak and promote mitotic spindle formation.Nature communications · 2015Article
- Nucleotide exchange in dimeric MCAK induces longitudinal and lateral stress at microtubule ends to support depolymerization.Structure (London, England : 1993) · 2014Article
- Kinetochore-microtubule stability governs the metaphase requirement for Eg5.Molecular biology of the cell · 2014Article
- Antagonistic spindle motors and MAPs regulate metaphase spindle length and chromosome segregation.Current biology : CB · 2013Article
- Review
- Cdk1 and Plk1 mediate a CLASP2 phospho-switch that stabilizes kinetochore-microtubule attachments.The Journal of cell biology · 2012Article
- Microtubule redistribution in growth cones elicited by focal inactivation of kinesin-5.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- MCAK activity at microtubule tips regulates spindle microtubule length to promote robust kinetochore attachment.The Journal of cell biology · 2012Article
- Regulation of localization and activity of the microtubule depolymerase MCAK.Bioarchitecture · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Accurate chromosome segregation during mitosis relies on the organization of microtubules into a bipolar spindle. Kinesin-5 proteins play an evolutionarily conserved role in establishing spindle bipolarity [1, 2] and clinical trials are currently evaluating inhibitors of human kinesin-5 (i.e., Eg5) for chemotherapeutic potential. However, in mammalian somatic cells, Eg5 activity is dispensable for maintenance of bipolar spindles once they are formed [3, 4], suggesting distinct requirements for establishment versus maintenance of spindle bipolarity. By combining Eg5 inhibition with RNA interference of other spindle proteins, we show that mitotic cells deficient in MCAK fail to maintain spindle bipolarity in the absence of Eg5 activity. Collapse of bipolar spindles in MCAK-deficient cells is driven by pole-focusing activities and is independent of MCAK function at centromeres, implicating hyperstabilized non-kinetochore microtubules in spindle collapse. Conversely, destabilizing nonkinetochore microtubules in early mitosis reduces the reliance on Eg5 for establishment of spindle bipolarity and renders cells partially resistant to Eg5 inhibitors. Thus, the temporal requirement for microtubule sliding generated by Eg5 activity during bipolar spindle assembly in mammalian cells is regulated by changes in the dynamic behavior of microtubules during mitosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.