ArticleBMC cancer2009
Impact of biospecimens handling on biomarker research in breast cancer.
Article in BMC cancer, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
49 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Molecular analysis of H&E- and Papanicolau-stained samples-systematic review.Histochemistry and cell biology · 2020Pooled it
- Immunosignature system for diagnosis of cancer.Proceedings of the National Academy of Sciences of the United States of America · 2014Trial
- Pre-analytical Best Practices for RNA Sequencing from Small Biopsies and Cytologic Specimens.Molecular diagnosis & therapy · 2026Review
- Building flexible and robust analysis frameworks for molecular subtyping of cancers.Molecular oncology · 2024Article
- Spatial epitranscriptomics reveals A-to-I editome specific to cancer stem cell microniches.Nature communications · 2022Article
- Improper preanalytical processes on peripheral blood compromise RNA quality and skew the transcriptional readouts of mRNA and LncRNA.Frontiers in genetics · 2022Article
- A review of coral bleaching specimen collection, preservation, and laboratory processing methods.PeerJ · 2021Article
- Endometrial Carcinoma, Grossing and Processing Issues: Recommendations of the International Society of Gynecologic Pathologists.International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists · 2019Article
- Audit of Quality and Quantity of Nucleic Acid Yield from Pediatric Acute Leukemia Cases Following a Bio-banking Initiative.Indian journal of hematology & blood transfusion : an official journal of Indian Society of Hematology and Blood Transfusion · 2019Article
- Differential effect of surgical manipulation on gene expression in normal breast tissue and breast tumor tissue.Molecular medicine (Cambridge, Mass.) · 2018Article
- Tumor Pre-Analytics in Molecular Pathology: Impact on Protein Expression and Analysis.Current pathobiology reports · 2018Review
- Brain tumor biobanking in the precision medicine era: building a high-quality resource for translational research in neuro-oncology.Neuro-oncology practice · 2017Review
- Protein purification and analysis: next generation Western blotting techniques.Expert review of proteomics · 2017Review
- Tissue alkalosis in cold-ischemia time.Scientific reports · 2017Article
- Effects of Cold Ischemia on Gene Expression: A Review and Commentary.Biopreservation and biobanking · 2016Review
- Automated measurement of estrogen receptor in breast cancer: a comparison of fluorescent and chromogenic methods of measurement.Laboratory investigation; a journal of technical methods and pathology · 2016Article
- Heterogeneity in global gene expression profiles between biopsy specimens taken peri-surgically from primary ER-positive breast carcinomas.Breast cancer research : BCR · 2016Article
- Nanoproteomic analysis of ischemia-dependent changes in signaling protein phosphorylation in colorectal normal and cancer tissue.Journal of translational medicine · 2016Article
- Biobanking in genomic medicine.Archives of pathology & laboratory medicine · 2015Review
- Critical roles of specimen type and temperature before and during fixation in the detection of phosphoproteins in breast cancer tissues.Laboratory investigation; a journal of technical methods and pathology · 2015Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundGene expression profiling is moving from the research setting to the practical clinical use. Gene signatures able to correctly identify high risk breast cancer patients as well as to predict response to treatment are currently under intense investigation. While technical issues dealing with RNA preparation, choice of array platforms, statistical analytical tools are taken into account, the tissue collection process is seldom considered. The time elapsed between surgical tissue removal and freezing of samples for biological characterizations is rarely well defined and/or recorded even for recently stored samples, despite the publications of standard operating procedures for biological sample collection for tissue banks.
methodsBreast cancer samples from 11 patients were collected immediately after surgical removal and subdivided into aliquots. One was immediately frozen and the others were maintained at room temperature for respectively 2, 6 and 24 hrs. RNA was extracted and gene expression profile was determined using cDNA arrays. Phosphoprotein profiles were studied in parallel.
resultsDelayed freezing affected the RNA quality only in 3 samples, which were not subjected to gene profiling. In the 8 breast cancer cases with apparently intact RNA also in sample aliquots frozen at delayed times, 461 genes were modulated simply as a function of freezing timing. Some of these genes were included in gene signatures biologically and clinically relevant for breast cancer. Delayed freezing also affected detection of phosphoproteins, whose pattern may be crucial for clinical decision on target-directed drugs.
conclusionTime elapsed between surgery and freezing of samples appears to have a strong impact and should be considered as a mandatory variable to control for clinical implications of inadequate tissue handling.
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