ArticleJournal of anatomy2009
Immunohistochemical detection of estrogen receptor beta in alveolar bone cells of estradiol-treated female rats: possible direct action of estrogen on osteoclast life span.
Article in Journal of anatomy, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 38 citations in OpenAlex.
- Combination of Pilose Antler Extract and Hydroxytyrosol Enhances Bone Mineral Density in Both Animals and Postmenopausal Women.Food science & nutrition · 2025Article
- Sex-dependent variation in bone adaptation: from degeneration to regeneration.Journal of orthopaedic translation · 2025Review
- The impact of estrogen on periodontal tissue integrity and inflammation-a mini review.Frontiers in dental medicine · 2025Review
- Efferocytosis and Bone Dynamics.Current osteoporosis reports · 2024Review
- Treatment with Raloxifene Induces the Expression of Kisspeptin, Insulin, and Androgen Receptors in Bones of Castrated Adult Female Rats.Revista brasileira de ortopedia · 2024Article
- Overview on postmenopausal osteoporosis and periodontitis: The therapeutic potential of phytoestrogens against alveolar bone loss.Frontiers in pharmacology · 2023Review
- Immunoexpression pattern of autophagy mediators in alveolar bone osteoclasts following estrogen withdrawal in female rats.Journal of molecular histology · 2021Article
- Recent Advances in Osteoclast Biological Behavior.Frontiers in cell and developmental biology · 2021Review
- Sexual Dimorphism in Osteoclasts.Cells · 2020Review
- Are Oxidative Stress and Inflammation Mediators of Bone Loss Due to Estrogen Deficiency? A Review of Current Evidence.Endocrine, metabolic & immune disorders drug targets · 2020Review
- The phytoestrogen genistein prevents trabecular bone loss and affects thyroid follicular cells in a male rat model of osteoporosis.Journal of anatomy · 2018Article
- Extra-gonadal sites of estrogen biosynthesis and function.BMB reports · 2016Review
- Estrogen receptors alpha and beta in bone.Bone · 2016Review
- Apoptosis and reduced microvascular density of the lamina propria during tooth eruption in rats.Journal of anatomy · 2015Article
- Biology of Bone Tissue: Structure, Function, and Factors That Influence Bone Cells.BioMed research international · 2015Review
- TGFβ inducible early gene-1 plays an important role in mediating estrogen signaling in the skeleton.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2014Article
- Increased apoptosis in osteoclasts and decreased RANKL immunoexpression in periodontium of cimetidine-treated rats.Journal of anatomy · 2013Article
- Structural and functional changes in the alveolar bone osteoclasts of estrogen-treated rats.Journal of anatomy · 2012Article
- Mast cells and MMP-9 in the lamina propria during eruption of rat molars: quantitative and immunohistochemical evaluation.Journal of anatomy · 2010Article
- Enhanced ERbeta immunoexpression and apoptosis in the germ cells of cimetidine-treated rats.Reproductive biology and endocrinology : RB&E · 2009Article
Corrections and comments
- Erratum issuedCruzoé-Souza, Mady [corrected to Crusodé de Souza, Mady]
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The role of estrogen in bone resorption has been specifically related to the effect of estrogen on the signalling pathway that inhibits the formation of osteoclasts. However, osteoclast apoptosis and a significant reduction in the number of these cells have been observed in the alveolar bone of female rats treated with estradiol. In the present study, the expression of estrogen receptor beta (ERbeta) in the cells of alveolar bone was evaluated in estradiol-treated and -untreated female rats. In order to test the possible direct action of estrogen on osteoclasts, the relationship between apoptosis and ERbeta expression in these cells was also analysed. The animals received estradiol for 14 days and the alveolar bone fragments were embedded in paraffin for the quantification of tartrate-resistant acid phosphatase-positive osteoclasts. The expression of ERbeta and apoptosis in the osteoclasts were evaluated by ERbeta immunohistochemistry and Terminal deoxynucleotidyl transferase-mediated dUTP Nick-End Labelling (TUNEL) methods, respectively. To confirm osteoclast death by apoptosis, these cells were analysed under transmission electron microscopy. Some osteoclasts from estradiol-treated animals were found to be undergoing apoptosis and the number of tartrate-resistant acid phosphatase-positive osteoclasts was significantly reduced. ERbeta immunolabelling was observed in the cytoplasm and nuclei of active osteoblasts, osteocytes and osteoclasts in both groups, suggesting a direct participation of estrogen on alveolar bone cells. However, following estradiol treatment, a strong ERbeta immunolabelling was often observed in the TUNEL-positive osteoclasts. Therefore, these results indicate that, in addition to the other signalling pathway, the reduction of alveolar bone resorption is also related to a direct action of estrogen on osteoclasts, promoting apoptosis in these cells, via ERbeta.
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