ArticleProceedings of the National Academy of Sciences of the United States of America2009
Trypsin is a multifunctional factor in spermatogenesis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 33 citations in OpenAlex.
- Cbx3a/HP1γ Deficiency Disrupts Meiotic Progression and Triggers Germ Cell Apoptosis in Nile Tilapia.Biomolecules · 2026Article
- Transcriptome Analysis of Transiently Reversible Cell Vacuolization Caused by Excessive Serum Concentration inBiology · 2024Article
- Towards Electrochemical Sensor Based on Molecularly Imprinted Polypyrrole for the Detection of Bacteria-Polymers · 2023Article
- Primary culture and endocrine functional analysis of Leydig cells in ducks (Frontiers in endocrinology · 2023Article
- An Antibacterial Peptide with High Resistance to Trypsin Obtained by Substituting d-Amino Acids for Trypsin Cleavage Sites.Antibiotics (Basel, Switzerland) · 2021Article
- Chemical and physical guidance of fish spermatozoa into the egg through the micropyle†,‡.Biology of reproduction · 2017Article
- Toward developing recombinant gonadotropin-based hormone therapies for increasing fertility in the flatfish Senegalese sole.PloS one · 2017Article
- Review
- Identification of differentially expressed genes in American cockroach ovaries and testes by suppression subtractive hybridization and the prediction of its miRNAs.Molecular genetics and genomics : MGG · 2013Article
- Partial characterization and ontogenetic development of pancreatic digestive enzymes in Japanese eel Anguilla japonica larvae.Fish physiology and biochemistry · 2013Article
- Toona sinensis Leaf Aqueous Extract Improves the Functions of Sperm and Testes via Regulating Testicular Proteins in Rats under Oxidative Stress.Evidence-based complementary and alternative medicine : eCAM · 2012Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Trypsin is well known as a pancreatic enzyme that is typically secreted into the intestine to digest proteins. We show in our current study, however, that trypsin is also a key factor in the control of spermatogenesis. A progestin in teleost fish, 17alpha, 20beta-dihydroxy-4-pregnen-3-one (DHP), is an essential component of the spermatogenesis pathway, particularly during the initiation of the first meiotic division. In the course of our investigations into the mechanisms underlying progestin-stimulated spermatogenesis, we identified that eel trypsinogen is upregulated in eel testis by DHP treatment. Trypsinogen is expressed in the Sertoli cells surrounding spermatogonia and in the membranes of spermatids and spermatozoa. Using an in vitro eel testicular culture system, we further analyzed the roles of trypsin in spermatogenesis. The inhibition of trypsin using specific antibodies or serine protease inhibitors was found to compromise DHP-induced spermatogenesis. A low dose of trypsin induces DNA synthesis and the expression of Spo11, a molecular marker of meiosis, in germ cells. By comparison, a higher dose of trypsin partially induced spermiogenesis. Furthermore, trypsin was detectable in the membranes of the spermatozoa and found to be associated with fertilization in fish. Our results thus demonstrate that trypsin and/or a trypsin-like protease is an essential and multifunctional factor in spermatogenesis.
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