ArticleRNA (New York, N.Y.)2010
Poly(A) signal-dependent degradation of unprocessed nascent transcripts accompanies poly(A) signal-dependent transcriptional pausing in vitro.
Article in RNA (New York, N.Y.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Transcription-dependent polyadenylation signal activity contributes to transcriptomic regulation during Forskolin-induced BeWo cell syncytialization.Frontiers in cell and developmental biology · 2026Article
- The yeast exoribonuclease Xrn1 and associated factors modulate RNA polymerase II processivity in 5' and 3' gene regions.The Journal of biological chemistry · 2020Article
- RNA surveillance by the nuclear RNA exosome: mechanisms and significance.Non-coding RNA · 2018Article
- Temporal Dissection of Rate Limiting Transcriptional Events Using Pol II ChIP and RNA Analysis of Adrenergic Stress Gene Activation.PloS one · 2015Article
- RBBP6 isoforms regulate the human polyadenylation machinery and modulate expression of mRNAs with AU-rich 3' UTRs.Genes & development · 2014Article
- Genome-wide mapping of yeast RNA polymerase II termination.PLoS genetics · 2014Article
- Disengaging polymerase: terminating RNA polymerase II transcription in budding yeast.Biochimica et biophysica acta · 2013Review
- A complex immunodeficiency is based on U1 snRNP-mediated poly(A) site suppression.The EMBO journal · 2012Article
- RNA polymerase II pausing downstream of core histone genes is different from genes producing polyadenylated transcripts.PloS one · 2012Article
- Unravelling the means to an end: RNA polymerase II transcription termination.Nature reviews. Molecular cell biology · 2011Review
- Mitosis in vertebrates: the G2/M and M/A transitions and their associated checkpoints.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2011Review
- A meiotic gene regulatory cascade driven by alternative fates for newly synthesized transcripts.Molecular biology of the cell · 2011Article
- A model in vitro system for co-transcriptional splicing.Nucleic acids research · 2010Article
- Coupled RNA polymerase II transcription and 3' end formation with yeast whole-cell extracts.RNA (New York, N.Y.) · 2010Article
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3 authors.
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Abstract
The poly(A) signal has long been known for its role in directing the cleavage and polyadenylation of eukaryotic mRNA. In recent years its additional coordinating role in multiple related aspects of gene expression has also become increasingly clear. Here we use HeLa nuclear extracts to study two of these activities, poly(A) signal-dependent transcriptional pausing, which was originally proposed as a surveillance checkpoint, and poly(A) signal-dependent degradation (PDD) of unprocessed transcripts from weak poly(A) signals. We confirm directly, by measuring the length of RNA within isolated transcription elongation complexes, that a newly transcribed poly(A) signal reduces the rate of elongation by RNA polymerase II and causes the accumulation of elongation complexes downstream from the poly(A) signal. We then show that if the RNA in these elongation complexes contains a functional but unprocessed poly(A) signal, degradation of the transcripts ensues. The degradation depends on the unprocessed poly(A) signal being functional, and does not occur if a mutant poly(A) signal is used. We suggest that during normal 3'-end processing the uncleaved poly(A) signal continuously samples competing reaction pathways for processing and for degradation, and that in the case of weak poly(A) signals, where poly(A) site cleavage is slow, the default pathway to degradation predominates.
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