ArticleBiologicals : journal of the International Association of Biological Standardization2010
Got black swimming dots in your cell culture? Identification of Achromobacter as a novel cell culture contaminant.
Article in Biologicals : journal of the International Association of Biological Standardization, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 25 citations in OpenAlex.
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- Analysis of bacterial community using pyrosequencing in semen from patients with chronic pelvic pain syndrome: a pilot study.Translational andrology and urology · 2020Article
- Prevalence of antibiotic resistance genes in cell culture liquid waste and the virulence assess for isolated resistant strains.Environmental science and pollution research international · 2019Article
- Exposure to different arsenic species drives the establishment of iron- and sulfur-oxidizing bacteria on rice root iron plaques.World journal of microbiology & biotechnology · 2019Article
- Laboratory contamination over time during low-biomass sample analysis.Molecular ecology resources · 2019Article
- Serendipitous crystallization and structure determination of bacterioferritin from Achromobacter.Acta crystallographica. Section F, Structural biology communications · 2018Article
- Social status shapes the bacterial and fungal gut communities of the honey bee.Scientific reports · 2018Article
- Human Cells Grown With or Without Substitutes for Fetal Bovine Serum.Cell medicine · 2018Article
- Sumoylation of Krüppel-like factor 4 inhibits pluripotency induction but promotes adipocyte differentiation.The Journal of biological chemistry · 2013Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Cell culture model systems are utilized for their ease of use, relative inexpensiveness, and potentially limitless sample size. Reliable results cannot be obtained, however, when cultures contain contamination. This report discusses the observation and identification of mobile black specks observed in multiple cell lines. Cultures of the contamination were grown, and DNA was purified from isolated colonies. The 16S rDNA gene was PCR amplified using primers that will amplify the gene from many genera, and then sequenced. Sequencing results matched the members of the genus Achromobacter, bacteria common in the environment. Achromobacter species have been shown to be resistant to multiple antibiotics. Attempts to decontaminate the eukaryotic cell culture used multiple antibiotics at different concentrations. The contaminating Achromobacter was eventually eliminated, without permanently harming the eukaryotic cells, using a combination of the antibiotics ciprofloxacin and piperacillin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.