ArticleAnalytical chemistry2009
Heparan sulfate separation, sequencing, and isomeric differentiation: ion mobility spectrometry reveals specific iduronic and glucuronic acid-containing hexasaccharides.
Article in Analytical chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Advanced Mass Spectrometry Techniques for the Characterization of Carbohydrates.Handbook of experimental pharmacology · 2025Review
- Mass Spectrometry-Based Techniques to Elucidate the Sugar Code.Chemical reviews · 2022Review
- Quantitative analysis of chondroitin sulfate disaccharides from human and rodent fixed brain tissue by electrospray ionization-tandem mass spectrometry.Glycobiology · 2019Article
- Characterization and Quantification of Highly Sulfated Glycosaminoglycan Isomers by Gated-Trapped Ion Mobility Spectrometry Negative Electron Transfer Dissociation MS/MS.Analytical chemistry · 2019Article
- Analysis of sulfates on low molecular weight heparin using mass spectrometry: structural characterization of enoxaparin.Expert review of proteomics · 2018Review
- Characterization of Isomeric Glycans by Reversed Phase Liquid Chromatography-Electronic Excitation Dissociation Tandem Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2018Article
- Isomer Information from Ion Mobility Separation of High-Mannose Glycan Fragments.Journal of the American Society for Mass Spectrometry · 2018Article
- MS/IR, a new MS-based hyphenated method for analysis of hexuronic acid epimers in glycosaminoglycans.Glycoconjugate journal · 2017Article
- Abnormally High Content of Free Glucosamine Residues Identified in a Preparation of Commercially Available Porcine Intestinal Heparan Sulfate.Analytical chemistry · 2016Article
- Travelling-wave ion mobility and negative ion fragmentation of high-mannose N-glycans.Journal of mass spectrometry : JMS · 2016Article
- Preparation and characterization of heparin hexasaccharide library with N-unsubstituted glucosamine residues.Glycoconjugate journal · 2015Article
- Discovery of a heparan sulfate 3-O-sulfation specific peeling reaction.Analytical chemistry · 2015Article
- High-field asymmetric-waveform ion mobility spectrometry and electron detachment dissociation of isobaric mixtures of glycosaminoglycans.Journal of the American Society for Mass Spectrometry · 2014Article
- Structure and response to flow of the glycocalyx layer.Biophysical journal · 2014Article
- Structural separations by ion mobility-MS for glycomics and glycoproteomics.Methods in molecular biology (Clifton, N.J.) · 2013Article
- Proteoglycan sequence.Molecular bioSystems · 2012Review
- Preparation, separation, and conformational analysis of differentially sulfated heparin octasaccharide isomers using ion mobility mass spectrometry.Analytical chemistry · 2012Article
- Differential Characterization and Classification of Tissue Specific Glycosaminoglycans by Tandem Mass Spectrometry and Statistical Methods.International journal of mass spectrometry · 2012Article
- Delineating diseases by IMS-MS profiling of serum N-linked glycans.Journal of proteome research · 2012Article
- Biologically Relevant Metal-Cation Binding Induces Conformational Changes in Heparin Oligosaccharides as Measured by Ion Mobility Mass Spectrometry.International journal of mass spectrometry · 2011Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
We describe the resolution of heparan sulfate (HS) isomers by chromatographic methods and their subsequent differentiation by mass spectrometry (MS), ion mobility, and (1)H nuclear magnetic resonance (NMR) analysis. The two purified hexasaccharide isomers produced nearly identical MS spectra, quantitative disaccharide profiles, and partial enzymatic digestions. However, both tandem spectrometry (MS(2)) and ion mobility spectrometry (IMS) indicated structural differences existed. All data suggested the distinction between the two hexasaccharides resided in their uronic acid stereochemistries. Glucuronic (GlcA) and iduronic acids (IdoA) were subsequently defined by (1)H NMR analysis completing the structural analysis and verifying the unique structures initially indicated by MS(2) and IMS. Our results suggest that IMS may be a powerful tool in the rapid differentiation of GlcA and IdoA containing isomers in the absence of prior structural knowledge.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.