ArticleDevelopmental dynamics : an official publication of the American Association of Anatomists2009
High resolution cell lineage tracing reveals developmental variability in leech.
Article in Developmental dynamics : an official publication of the American Association of Anatomists, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 27 citations in OpenAlex.
- Transgenesis enables mapping of segmental ganglia in the leech Helobdella austinensis.The Journal of experimental biology · 2024Article
- Pax3/7 regulates neural tube closure and patterning in a non-vertebrate chordate.Frontiers in cell and developmental biology · 2022Article
- Ebf Activates Expression of a Cholinergic Locus in a Multipolar Motor Ganglion Interneuron Subtype inFrontiers in neuroscience · 2021Article
- Effector gene expression underlying neuron subtype-specific traits in the Motor Ganglion of Ciona.Developmental biology · 2020Article
- Maps of variability in cell lineage trees.PLoS computational biology · 2019Article
- Cell lineage and cell cycling analyses of the 4d micromere using live imaging in the marine annelideLife · 2017Article
- Fine taxonomic sampling of nervous systems within Naididae (Annelida: Clitellata) reveals evolutionary lability and revised homologies of annelid neural components.Frontiers in zoology · 2015Article
- Cleavage pattern and fate map of the mesentoblast, 4d, in the gastropod Crepidula: a hallmark of spiralian development.EvoDevo · 2012Article
- Intermediate filament genes as differentiation markers in the leech Helobdella.Development genes and evolution · 2011Article
- A new molecular logic for BMP-mediated dorsoventral patterning in the leech Helobdella.Current biology : CB · 2011Article
- Lineage analysis of micromere 4d, a super-phylotypic cell for Lophotrochozoa, in the leech Helobdella and the sludgeworm Tubifex.Developmental biology · 2011Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Knowing the normal patterns of embryonic cell proliferation, migration, and differentiation is a cornerstone for understanding development. Yet for most species, the precision with which embryonic cell lineages can be determined is limited by technical considerations (the large numbers of cells, extended developmental times, opacity of the embryos), and these are exacerbated by the inherent variability of the lineages themselves. Here, we present an improved method of cell lineage tracing in the leech Helobdella, driving the expression of a nuclearly localized histone H2B:GFP (green fluorescent protein) fusion protein in selected lineages by microinjection of a plasmid vector. This construct generates a long lasting and minimally mosaic signal with single cell resolution, and does not disrupt the development of most lineages tested. We have validated this technique by elucidating details of cell lineages contributing to segmental and prostomial tissues that could not be observed with standard dextran lineage tracers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.