ArticleProceedings of the National Academy of Sciences of the United States of America1991
Accurate and efficient RNA polymerase II transcription with a soluble nuclear fraction derived from Drosophila embryos.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed.
- Real-time visualization of reconstituted transcription reveals RNAPII activation mechanisms at single promoters.Cell reports · 2025Article
- Real-time visualization of reconstituted transcription reveals RNA polymerase II activation mechanisms at single promoters.bioRxiv : the preprint server for biology · 2025Article
- Mechanisms and Functions of the RNA Polymerase II General Transcription Machinery during the Transcription Cycle.Biomolecules · 2024Review
- Functionally distinct promoter classes initiate transcription via different mechanisms reflected in focused versus dispersed initiation patterns.The EMBO journal · 2023Article
- Article
- A Developmental Program Truncates Long Transcripts to Temporally Regulate Cell Signaling.Developmental cell · 2018Article
- NDF, a nucleosome-destabilizing factor that facilitates transcription through nucleosomes.Genes & development · 2018Article
- Single molecule microscopy reveals mechanistic insight into RNA polymerase II preinitiation complex assembly and transcriptional activity.Nucleic acids research · 2016Article
- SMARCAD1 is an ATP-dependent stimulator of nucleosomal H2A acetylation via CBP, resulting in transcriptional regulation.Scientific reports · 2016Article
- Two new insulator proteins, Pita and ZIPIC, target CP190 to chromatin.Genome research · 2015Article
- RNA polymerase III accurately initiates transcription from RNA polymerase II promoters in vitro.The Journal of biological chemistry · 2014Article
- Article
- Differential utilization of TATA box-binding protein (TBP) and TBP-related factor 1 (TRF1) at different classes of RNA polymerase III promoters.The Journal of biological chemistry · 2013Article
- Article
- In vitro transcription and immobilized template analysis of preinitiation complexes.Current protocols in molecular biology · 2012Article
- Polycomb purification by in vivo biotinylation tagging reveals cohesin and Trithorax group proteins as interaction partners.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Metazoan Scc4 homologs link sister chromatid cohesion to cell and axon migration guidance.PLoS biology · 2006Article
- High mobility group proteins HMGD and HMGZ interact genetically with the Brahma chromatin remodeling complex in Drosophila.Genetics · 2006Article
- Identification of a Drosophila Myb-E2F2/RBF transcriptional repressor complex.Genes & development · 2004Article
- The neuron-specific RNA-binding protein ELAV regulates neuroglian alternative splicing in neurons and binds directly to its pre-mRNA.Genes & development · 2001Article
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3 authors.
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Abstract
We describe the preparation and biochemical properties of a soluble nuclear fraction derived from Drosophila embryos. This extract, which can be easily prepared in 2.5 hr, is capable of accurate and efficient RNA polymerase II transcription of a variety of diverse genes from both Drosophila and mammals. With the relatively strong promoter of the Drosophila Krüppel gene, it is possible to achieve 20% template usage in a single round of transcription, which is considerably higher than the template usage of approximately 3% seen with standard nuclear extracts. Further, although U small nuclear RNA genes are refractory to transcription with HeLa transcription extracts, the soluble nuclear fraction transcribes a U1 small nuclear RNA gene from Drosophila. Moreover, transcriptional activation by sequence-specific activators can be attained in vitro with the soluble nuclear fraction. The overall transcriptional efficiency appears limited to 0.45 transcript per template of DNA per 30 min, but the mechanism of limitation is not known. The soluble nuclear fraction, which was developed to recreate the environment within the nucleus, should be useful when high efficiencies of RNA polymerase II transcription are desired.
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