ArticlePloS one2009
The peculiarities of large intron splicing in animals.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 74 citations in OpenAlex.
- Emerging Functions of the Repeat Genome in Nuclear Structure: A View from the Human Karyotype.Annual review of genomics and human genetics · 2025Review
- Profiling transcriptome composition and dynamics within nuclear compartments using SLAM-RT&Tag.Molecular cell · 2025Article
- Genome-wide identification of CAMTA genes and their expression dependence on light and calcium signaling during seedling growth and development in mung bean.BMC genomics · 2024Article
- Genes divided according to the relative position of the longest intron show increased representation in different KEGG pathways.BMC genomics · 2024Article
- Co-transcriptional splicing facilitates transcription of gigantic genes.PLoS genetics · 2024Article
- Recursive splicing discovery using lariats in total RNA sequencing.Life science alliance · 2023Article
- Estimating transcriptome complexities across eukaryotes.BMC genomics · 2023Article
- Novel mechanisms for the synthesis of important secondary metabolites inFrontiers in plant science · 2023Article
- Introns: the "dark matter" of the eukaryotic genome.Frontiers in genetics · 2023Review
- Analysis of Common SNPs across Continents Reveals Major Genomic Differences between Human Populations.Genes · 2022Article
- Nascent RNA scaffolds contribute to chromosome territory architecture and counter chromatin compaction.Molecular cell · 2021Article
- HNRNPM controls circRNA biogenesis and splicing fidelity to sustain cancer cell fitness.eLife · 2021Article
- Transcriptomes of an Array of Chicken Ovary, Intestinal, and Immune Cells and Tissues.Frontiers in genetics · 2021Article
- Satellite DNA-containing gigantic introns in a unique gene expression program during Drosophila spermatogenesis.PLoS genetics · 2019Article
- Landscape of alternative splicing in Capra_hircus.Scientific reports · 2018Article
- CRISPR/Cas9-mediated genome editing induces exon skipping by complete or stochastic altering splicing in the migratory locust.BMC biotechnology · 2018Article
- RNA biology of disease-associated microsatellite repeat expansions.Acta neuropathologica communications · 2017Review
- Circular RNAs-one of the enigmas of the brain.Neurogenetics · 2017Review
- Cutting a Long Intron Short: Recursive Splicing and Its Implications.Frontiers in physiology · 2016Article
- Non-sequential and multi-step splicing of the dystrophin transcript.RNA biology · 2016Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In mammals a considerable 92% of genes contain introns, with hundreds and hundreds of these introns reaching the incredible size of over 50,000 nucleotides. These "large introns" must be spliced out of the pre-mRNA in a timely fashion, which involves bringing together distant 5' and 3' acceptor and donor splice sites. In invertebrates, especially Drosophila, it has been shown that larger introns can be spliced efficiently through a process known as recursive splicing-a consecutive splicing from the 5'-end at a series of combined donor-acceptor splice sites called RP-sites. Using a computational analysis of the genomic sequences, we show that vertebrates lack the proper enrichment of RP-sites in their large introns, and, therefore, require some other method to aid splicing. We analyzed over 15,000 non-redundant, large introns from six mammals, 1,600 from chicken and zebrafish, and 560 non-redundant large introns from five invertebrates. Our bioinformatic investigation demonstrates that, unlike the studied invertebrates, the studied vertebrate genomes contain consistently abundant amounts of direct and complementary strand interspersed repetitive elements (mainly SINEs and LINEs) that may form stems with each other in large introns. This examination showed that predicted stems are indeed abundant and stable in the large introns of mammals. We hypothesize that such stems with long loops within large introns allow intron splice sites to find each other more quickly by folding the intronic RNA upon itself at smaller intervals and, thus, reducing the distance between donor and acceptor sites.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.