ArticleJournal of clinical microbiology2010
Diagnosis of congenital toxoplasmosis by using a whole-blood gamma interferon release assay.
Article in Journal of clinical microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 58 citations in OpenAlex.
- In defense of children's brain: reshuffling the laboratory toolbox for the diagnosis of congenital toxoplasmosis.Journal of clinical microbiology · 2024Article
- Ocular toxoplasmosis: a review of the current diagnostic and therapeutic approaches.International ophthalmology · 2022Review
- Interferon-γ and IL-10 Release Assay for Patients with Ocular Toxoplasmosis.The American journal of tropical medicine and hygiene · 2020Article
- Putative biomarkers for early diagnosis and prognosis of congenital ocular toxoplasmosis.Scientific reports · 2020Article
- Serological diagnosis of Toxoplasma gondii: analysis of false-positive IgG results and implications.Parasite (Paris, France) · 2020Article
- Toxoplasmosis in Germany.Deutsches Arzteblatt international · 2019Review
- Secretory Microneme Proteins Induce T-Cell Recall Responses in Mice Chronically Infected with Toxoplasma gondii.mSphere · 2019Article
- Maternal and Congenital Toxoplasmosis: Diagnosis and Treatment Recommendations of a French Multidisciplinary Working Group.Pathogens (Basel, Switzerland) · 2019Article
- Solid Organ Transplant and Parasitic Diseases: A Review of the Clinical Cases in the Last Two Decades.Pathogens (Basel, Switzerland) · 2018Review
- A T-cell diagnostic test for cystic echinococcosis based on Antigen B peptides.Parasite immunology · 2017Article
- Interferon-Gamma Release Assay: An Effective Tool to Detect Early Toxoplasma gondii Infection in Mice.PloS one · 2015Article
- Synergistic antigen combinations for the development of interferon gamma release assays for paucibacillary leprosy.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2014Article
- Immunity to cytomegalovirus in early life.Frontiers in immunology · 2014Review
- Epidemiology of and diagnostic strategies for toxoplasmosis.Clinical microbiology reviews · 2012Review
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Congenital toxoplasmosis in newborns is generally subclinical, but infected infants are at risk of developing ocular lesions. Diagnosis at birth relies mainly on serological tests. Cell-mediated immunity plays the major role in resistance to infection but is not routinely investigated for diagnostic purposes. Here, we describe a simple test based on the gamma interferon (IFN-gamma) response after stimulation of whole blood by crude parasitic antigens. One milliliter of heparinized blood was centrifuged; plasma was kept for routine serological tests, and pellets were resuspended in culture medium. After 24 h of culture in the presence of crude Toxoplasma gondii antigen, the cells were centrifuged and the supernatant was assayed for IFN-gamma. For 62 infants under 1 year of age born to mothers who were infected during pregnancy, the sensitivity and specificity of the test were 94% (with positive results for 16 of 17 infected infants) and 98% (with negative results for 44 of 45 uninfected infants), respectively. The false-negative result was for a treated baby who gave positive results after the withdrawal of treatment. The false positive was obtained for a 3-month-old baby. For a cohort of 124 congenitally infected patients between 1 and 30 years of age, the sensitivity of the assay was 100%. We present a simple test based on IFN-gamma secretion to assess cell-mediated immunity in toxoplasmosis. As only 1 ml of blood is required to investigate humoral and cellular immunity, our assay is well adapted for the study of congenital toxoplasmosis in infants. Using purified antigens or recombinant peptides may improve the test performance.
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