ArticleAmerican journal of physiology. Renal physiology2010
Heterogeneity of H-K-ATPase-mediated acid secretion along the mouse collecting duct.
Article in American journal of physiology. Renal physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 24 citations in OpenAlex.
- Correlating metabolites, lipids, and N-glycans in murine kidney microstructures via integrative mass spectrometry imaging.Nature communications · 2026Article
- Epac2 Deficiency Compromises Adaptation to Dietary Acidification by Decreasing H+ Transport in the Renal Nephron.Function (Oxford, England) · 2025Article
- TRPV4 expression in the renal tubule is necessary for maintaining whole body KAmerican journal of physiology. Renal physiology · 2023Article
- Review
- Impaired Renal HCOJournal of the American Society of Nephrology : JASN · 2020Article
- Transcriptome analysis of the growth performance of hybrid mandarin fish after food conversion.PloS one · 2020Article
- Deletion of the serine protease CAP2/Tmprss4 leads to dysregulated renal water handling upon dietary potassium depletion.Scientific reports · 2019Article
- TRPV4 deletion protects against hypokalemia during systemic KAmerican journal of physiology. Renal physiology · 2019Article
- An unexpected journey: conceptual evolution of mechanoregulated potassium transport in the distal nephron.American journal of physiology. Cell physiology · 2016Review
- Cell-specific regulation of L-WNK1 by dietary K.American journal of physiology. Renal physiology · 2016Article
- Molecular mechanisms and regulation of urinary acidification.Comprehensive Physiology · 2014Review
- Endothelin-1 inhibits sodium reabsorption by ET(A) and ET(B) receptors in the mouse cortical collecting duct.American journal of physiology. Renal physiology · 2013Article
- Pyk2 regulates H+-ATPase-mediated proton secretion in the outer medullary collecting duct via an ERK1/2 signaling pathway.American journal of physiology. Renal physiology · 2012Article
- Luminal flow modulates H+-ATPase activity in the cortical collecting duct (CCD).American journal of physiology. Renal physiology · 2012Article
- Role of NKCC in BK channel-mediated net K⁺ secretion in the CCD.American journal of physiology. Renal physiology · 2011Article
- Mineralocorticoids stimulate the activity and expression of renal H+,K+-ATPases.Journal of the American Society of Nephrology : JASN · 2011Article
- Pharmacological profiles of the murine gastric and colonic H,K-ATPases.Biochimica et biophysica acta · 2010Article
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6 authors at 2 institutions in 1 country.
Funding
Abstract
In the collecting duct (CD), H-K-ATPases function in cation reabsorption and H secretion. This study evaluated H-K-ATPase-mediated H secretion along the mouse CD, measured as EIPA- and luminal bafilomycin A(1)-insensitive intracellular pH (pH(i)) recovery from acute H loading (NH(4)) using BCECF. pH(i) recovery was measured in 1) microperfused cortical, outer medullary, and inner medullary CDs (CCD, OMCD, and IMCD) from C57BL/6J mice fed a normal diet and 2) common murine CD cell lines. H-K-ATPase activity along the native, microperfused CD was greatest in the CCD, less in the OMCD, and least in the IMCD (0.10 +/- 0.02, 0.04 +/- 0.01, and 0.01 +/- 0.002 U/min, respectively). H-K-ATPase activity was 0.30 +/- 0.03 and 0.26 +/- 0.03 in A- and B-type ICs, respectively, and was sensitive to Sch-28080 or ouabain. pH(i) recovery was greatest in the OMCD(1) cell line (0.25 +/- 0.01) and less in mpkCCD(c14) (0.17 +/- 0.01), mIMCD-K2 (0.12 +/- 0.01), and mIMCD-3 (0.05 +/- 0.01) cells. EIPA inhibited the majority of pH(i) recovery in these cells (100%, 64%, 75%, and 80% in mpkCCD(c14), OMCD(1), mIMCD-K2, and mIMCD-3, respectively). In OMCD(1) cells, where EIPA-insensitive pH(i) recovery was greatest, H-K-ATPase activity was 0.10 +/- 0.01 and was significantly inhibited (80%) by Sch-28080. We conclude that 1) H-K-ATPase-mediated H secretion in the native mouse CD is greatest in the ICs of the CCD, 2) A- and B-type ICs possess HKalpha(1) and HKalpha(2) H-K-ATPase activity, and 3) the OMCD(1) cell line best exhibits H-K-ATPase.
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