ArticleParasites & vectors2009
Distribution of Brugia malayi larvae and DNA in vector and non-vector mosquitoes: implications for molecular diagnostics.
Article in Parasites & vectors, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed.
- Post elimination of lymphatic filariasis: a situation analysis of brugian filariasis and vector potentialities within the filarial transmission belt in Sri Lanka.Parasites & vectors · 2026Article
- Impact ofTropical medicine and infectious disease · 2025Article
- Brugia malayi and other filarial parasite species in animals in areas endemic for lymphatic filariasis in Belitung District, Indonesia.PLoS neglected tropical diseases · 2025Article
- Preliminary data on the vector competence of Aedes caspius for Dirofililaria immitis in a traditionally endemic area of northern Italy.Parasites & vectors · 2025Article
- Detection of Wuchereria bancrofti DNA in wild caught vector and non-vector mosquitoes: implications for elimination of lymphatic filariasis.Molecular biology reports · 2024Article
- Nondestructive Methods of Pathogen Detection: Importance of Mosquito Integrity in Studies of Disease Transmission and Control.Pathogens (Basel, Switzerland) · 2023Review
- No evidence of lymphatic filariasis transmission in Bamako urban setting after three mass drug administration rounds.Parasitology research · 2022Article
- The use of molecular xenomonitoring for surveillance of mosquito-borne diseases.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2021Review
- Field evaluation of DNA detection of human filarial and malaria parasites using mosquito excreta/feces.PLoS neglected tropical diseases · 2020Article
- Searching for the proverbial needle in a haystack: advances in mosquito-borne arbovirus surveillance.Parasites & vectors · 2018Review
- A superhydrophobic cone to facilitate the xenomonitoring of filarial parasites, malaria, and trypanosomes using mosquito excreta/feces.Gates open research · 2017Article
- First molecular identification of Dirofilaria spp. (Onchocercidae) in mosquitoes from Serbia.Parasitology research · 2016Article
- A Novel Xenomonitoring Technique Using Mosquito Excreta/Feces for the Detection of Filarial Parasites and Malaria.PLoS neglected tropical diseases · 2016Article
- Is molecular xenomonitoring of mosquitoes for Dirofilaria repens suitable for dirofilariosis surveillance in endemic regions?Parasitology research · 2016Article
- Molecular xenomonitoring using mosquitoes to map lymphatic filariasis after mass drug administration in American Samoa.PLoS neglected tropical diseases · 2014Article
- Midgut barrier imparts selective resistance to filarial worm infection in Culex pipiens pipiens.PLoS neglected tropical diseases · 2010Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
backgroundThe purpose of this study was to extend prior studies of molecular detection of Brugia malayi DNA in vector (Aedes aegypti- Liverpool) and non-vector (Culex pipiens) mosquitoes at different times after ingestion of infected blood.
resultsParasite DNA was detected over a two week time course in 96% of pooled thoraces of vector mosquitoes. In contrast, parasite DNA was detected in only 24% of thorax pools from non-vectors; parasite DNA was detected in 56% of midgut pools and 47% of abdomen pools from non-vectors. Parasite DNA was detected in vectors in the head immediately after the blood meal and after 14 days. Parasite DNA was also detected in feces and excreta of the vector and non-vector mosquitoes which could potentially confound results obtained with field samples. However, co-housing experiments failed to demonstrate transfer of parasite DNA from infected to non-infected mosquitoes. Parasites were also visualized in mosquito tissues by immunohistololgy using an antibody to the recombinant filarial antigen Bm14. Parasite larvae were detected consistently after mf ingestion in Ae. aegypti- Liverpool. Infectious L3s were seen in the head, thorax and abdomen of vector mosquitoes 14 days after Mf ingestion. In contrast, parasites were only detected by histology shortly after the blood meal in Cx. pipiens, and these were not labeled by the antibody.
conclusionThis study provides new information on the distribution of filarial parasites and parasite DNA in vector and non-vector mosquitoes. This information should be useful for those involved in designing and interpreting molecular xenomonitoring studies.
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Registered trials
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