ArticleInternational journal of colorectal disease2010
Influence of NFkappaB inhibitors on IL-1beta-induced chemokine CXCL8 and -10 expression levels in intestinal epithelial cell lines: glucocorticoid ineffectiveness and paradoxical effect of PDTC.
Article in International journal of colorectal disease, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 22 citations in OpenAlex.
- Enhanced IGFL1 translation in response to IL-1β is controlled by distinct 3'UTR elements.PloS one · 2026Article
- IL-1β-Induced CXCL10 Expression in THP-1 Monocytic Cells Involves the JNK/c-Jun and NF-κB-Mediated Signaling.Pharmaceuticals (Basel, Switzerland) · 2024Article
- Platelet-Rich Fibrin Increases CXCL8 Expression in Gingival Fibroblasts.Biomedicines · 2024Article
- Photobiomodulation reduces neuropathic pain after spinal cord injury by downregulating CXCL10 expression.CNS neuroscience & therapeutics · 2023Article
- Ferroptosis: A Trigger of Proinflammatory State Progression to Immunogenicity in Necroinflammatory Disease.Frontiers in immunology · 2021Review
- Glucocorticoid receptor modulates dendritic cell function in ulcerative colitis.Histology and histopathology · 2020Review
- N-Acetyl-Seryl-Aspartyl-Lysyl-Proline Mitigates Experimental Colitis Through Inhibition of Intestinal Mucosal Inflammatory ResponsesFrontiers in pharmacology · 2020Article
- RNA-Seq analysis of ileocecal valve and peripheral blood from Holstein cattle infected with Mycobacterium avium subsp. paratuberculosis revealed dysregulation of the CXCL8/IL8 signaling pathway.Scientific reports · 2019Article
- Neisseria gonorrhoeae uses cellular proteins CXCL10 and IL8 to enhance HIV-1 transmission across cervical mucosa.American journal of reproductive immunology (New York, N.Y. : 1989) · 2019Article
- Role of the CXCL8-CXCR1/2 Axis in Cancer and Inflammatory Diseases.Theranostics · 2017Review
- ROCK activity affects IL-1-induced signaling possibly through MKK4 and p38 MAPK in Caco-2 cells.In vitro cellular & developmental biology. Animal · 2016Article
- Hydrogen sulfide from a NaHS source attenuates dextran sulfate sodium (DSS)-induced inflammation via inhibiting nuclear factor-κB.Journal of Zhejiang University. Science. B · 2016Article
- Article
- Glibenclamide reduces pro-inflammatory cytokine production by neutrophils of diabetes patients in response to bacterial infection.Scientific reports · 2013Article
- NF-κΒ inhibition is ineffective in blocking cytokine-induced IL-8 production but P38 and STAT1 inhibitors are effective.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2012Article
- Therapeutic effects of pyrrolidine dithiocarbamate on acute lung injury in rabbits.Journal of translational medicine · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeActivation of intestinal epithelial cell (IEC) nuclear factor kappaB (NFkappaB) and the consequent chemokine upregulation are crucial events in inflammatory bowel disease (IBD) pathogenesis. Not much is known about the consequences of NFkappaB inhibition in terms of chemokine expression in intestinal cells. Therefore, we aimed to evaluate the efficacy of compounds known to disrupt the NFkappaB pathway on NFkappaB transcriptional activity and CXCL8 and CXCL10 gene expression in intestinal cell lines.
methodsThe influence of NFkappaB inhibitors (dexamethasone, pyrrolidine dithiocarbamate (PDTC) and BAY 11-7082) on IL-1beta-induced NFkappaB transcriptional activity was investigated by transient transfection of Caco-2 cells with an NFkappaB-secreted alkaline phosphatase reporter plasmid. Il-1beta stimulated CXCL8 and CXCL10 mRNA and protein expression and was studied in Caco-2 and HT29 cells in the presence and absence of the NFkappaB inhibitors by quantitative real-time polymerase chain reaction and enzyme-linked immunosorbent serologic assay, respectively. To reveal alternative signalling cascades, experiments were also performed in the presence of the p38MAPK inhibitor SB 203580 and the ERK inhibitor PD 98059.
resultsDexamethasone did not downregulate chemokine expression sufficiently, probably due to a lack of glucocorticoid receptors in these cells. While BAY11-7082 inhibited chemokine expression, PDTC led to a paradoxical upregulation of CXCL8 in Caco-2 cells, which could be prevented by inhibition of p38MAPK.
conclusionThese data explain the frequent unresponsiveness of IBD to glucocorticoid treatment and suggest that alternative NFkappaB inhibition in IECs might be of use in IBD therapy. Drug development based on measuring anti-NFkappaB activity might be misleading and should therefore also include studies on relevant gene products.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.