ArticleEuropean biophysics journal : EBJ2010
Kinesin velocity increases with the number of motors pulling against viscoelastic drag.
Article in European biophysics journal : EBJ, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed.
- Multiple kinesins speed up cargo transport in crowded environments by sharing load.Communications biology · 2025Article
- The Impact of Rate Formulations on Stochastic Molecular Motor Dynamics.Scientific reports · 2019Article
- Geometry of antiparallel microtubule bundles regulates relative sliding and stalling by PRC1 and Kif4A.eLife · 2018Article
- Single Molecule Studies Enabled by Model-Based Controller Design.IEEE/ASME transactions on mechatronics : a joint publication of the IEEE Industrial Electronics Society and the ASME Dynamic Systems and Control Division · 2018Article
- Kinesin and Dynein Mechanics: Measurement Methods and Research Applications.Journal of biomechanical engineering · 2018Review
- Transport efficiency of membrane-anchored kinesin-1 motors depends on motor density and diffusivity.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Dual-Color Herpesvirus Capsids Discriminate Inoculum from Progeny and Reveal Axonal Transport Dynamics.Journal of virology · 2016Article
- Self-propelling vesicles define glycolysis as the minimal energy machinery for neuronal transport.Nature communications · 2016Article
- A nonequilibrium power balance relation for analyzing dissipative filament dynamics.The European physical journal. E, Soft matter · 2015Article
- A method for multiprotein assembly in cells reveals independent action of kinesins in complex.The Journal of cell biology · 2014Article
- Motor coupling through lipid membranes enhances transport velocities for ensembles of myosin Va.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Microtubules are required for efficient epithelial tight junction homeostasis and restoration.American journal of physiology. Cell physiology · 2014Article
- Article
- Kinesin-1 and mitochondrial motility control by discrimination of structurally equivalent but distinct subdomains in Ran-GTP-binding domains of Ran-binding protein 2.Open biology · 2013Article
- Regulation of ASIC channels by a stomatin/STOML3 complex located in a mobile vesicle pool in sensory neurons.Open biology · 2012Article
- Molecular motor function in axonal transport in vivo probed by genetic and computational analysis in Drosophila.Molecular biology of the cell · 2012Article
- Force-velocity relationship for multiple kinesin motors pulling a magnetic bead.European biophysics journal : EBJ · 2011Article
- Effects of surface passivation on gliding motility assays.PloS one · 2011Article
- Magnet polepiece design for uniform magnetic force on superparamagnetic beads.The Review of scientific instruments · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Although the properties of single kinesin molecular motors are well understood, it is not clear whether multiple motors pulling a single vesicle in a cell cooperate or interfere with one another. To learn how small numbers of motors interact, microtubule gliding assays were carried out with full-length Drosophila kinesin in a novel motility medium containing xanthan, a stiff, water-soluble polysaccharide. At 2 mg/ml xanthan, the zero-shear viscosity of this medium is 1,000 times the viscosity of water, similar to cellular viscosity. To mimic the rheological drag force on the motors when attached to a vesicle in a cell, we attached a 2 microm bead to one end of the microtubule (MT). During gliding assays in our novel medium, the moving bead exerted a drag force of 4-15 pN on the kinesins pulling the MT. The velocity of MTs with an attached bead increased with MT length and with kinesin concentration. The increase with MT length arose because the number of motors is directly proportional to MT length. Our results show that small numbers of kinesins cooperate constructively when pulling against a viscoelastic drag. In the absence of a bead but still in the viscous medium, MT velocity was independent of MT length and kinesin concentration because the thin MT, like a snake moving through grass, was able to move between xanthan molecules with little resistance. A minimal shared-load model in which the number of motors is proportional to MT length fits the observed dependence of gliding velocity on MT length and kinesin concentration.
Indexed as
Identifiers
19921171What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.